| Diversity and distribution of the prokaryotic community in near-surface permafrost sediments in the Tianshan Mountains, China | |
| Yang, Daqun1; Wang, Jianhui2; Bai, Yu1; Xu, Shijian1; An, Lizhe1 | |
| 刊名 | CANADIAN JOURNAL OF MICROBIOLOGY
 |
| 2008-04-01 | |
| 卷号 | 54期号:4页码:270-280 |
| 关键词 | alpine permafrost community structure phylogenetic diversity DGGE |
| ISSN号 | 0008-4166 |
| DOI | 10.1139/W08-004 |
| 通讯作者 | An, Lizhe(lizhean@hotmail.com) |
| 英文摘要 | The community structures and diversity of bacteria and archaea were investigated at 4 depths (1.5, 2.0, 2.5, and 3.0 m) in permafrost sediments in the Tianshan Mountains, using denaturing gradient gel electrophoresis of 16S rRNA gene amplified by polymerase chain reaction. Phylogenetic analysis of the dominant bands sequenced revealed the presence of rich diversity of bacteria, which could be related to the Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Bacteroidetes, Firmicutes, and Chloroflexi. The Proteobacteria, consisting of the alpha, beta, gamma, and epsilon subdivisions, were clearly the dominant group at all depths studied. Archaeal diversity was relatively low and archaeal 16S rRNA gene sequences were grouped into 3 phylogenetic clusters within the 2 kingdoms Euryarchaeota and Crenarchaeota. Within the Euryarchaeota, methanogen-related group II was most abundant at shallow depth (1.5 m), whereas halobacterium-related group I dominated at greater depths. The low-temperature Crenarchaeota group was detected only at 2.5 and 3.0 m. Specific-depth distribution of methanogen-related Euryarchaeota group II and denitrifying bacteria of the genus Pseudomonas dominated at 1.5 m depth, accompanied by a distinct peak in the ratio of NH4-N to NO3/NO2-N, implying the potential capacity of these organisms in near-surface permafrost to release the greenhouse gases N2O and CH4. |
| 收录类别 | SCI |
| WOS关键词 | AUTOTROPHIC CO2 FIXATION ; 16S RIBOSOMAL-RNA ; PHYLOGENETIC DIVERSITY ; EPSILON-PROTEOBACTERIA ; MICROBIAL DIVERSITY ; BACTERIAL COMMUNITY ; SALINITY GRADIENT ; SOIL ; COLD ; CRENARCHAEOTA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| 语种 | 英语 |
| 出版者 | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS |
| WOS记录号 | WOS:000255040200004 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2556034 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Daqun,Wang, Jianhui,Bai, Yu,et al. Diversity and distribution of the prokaryotic community in near-surface permafrost sediments in the Tianshan Mountains, China[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2008,54(4):270-280. |
| APA | Yang, Daqun,Wang, Jianhui,Bai, Yu,Xu, Shijian,&An, Lizhe.(2008).Diversity and distribution of the prokaryotic community in near-surface permafrost sediments in the Tianshan Mountains, China.CANADIAN JOURNAL OF MICROBIOLOGY,54(4),270-280. |
| MLA | Yang, Daqun,et al."Diversity and distribution of the prokaryotic community in near-surface permafrost sediments in the Tianshan Mountains, China".CANADIAN JOURNAL OF MICROBIOLOGY 54.4(2008):270-280. |
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