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	Variation in the ovine PRKAG3 gene
	Yang, Guo1,2; Zhou, Huitong2; Wang, Ruoyu1; Hickford, Jon2
刊名	GENE
	2015-08-10
卷号	567期号:2页码:251-254
关键词	PRKAG3 Genetic variation PCR-SSCP Sheep Nucleotide substitution Amino acid substitution
ISSN号	0378-1119
DOI	10.1016/j.gene.2015.05.021
通讯作者	Wang, Ruoyu(wangruoyu@lzb.ac.cn)
英文摘要	The 5'AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that controls cellular energy homeostasis in response to environmental or nutritional stress. The PRKAG3 gene (PRICAG3) encodes the gamma 3 subunit of the AMPK. Variation in this gene has been found to be associated with meat quality traits in pigs. In this study, we used polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) to investigate variation in exon 3 and exons 4-6 of ovine PRKAG3. In 160 New Zealand Suffolk sheep, two variant sequences (named a and b) were identified in the exon 3 region of the gene and three variant sequences (named A, B and C) were identified in the exon 4-6 region of the gene, respectively. A total of three nucleotide substitutions were revealed and these were located in intron 4, exon 4 and intron 5, respectively. The nucleotide substitution identified in the exon 4 (g.2656 C>T) could nominally lead to an amino acid substitution of tryptophan to arginine at position 230 (R230W) in ovine PRKAG3. In comparison with the PRKAG3 amino add sequences in other species, this R230W substitution appeared to occur only in sheep. This is the first report of genetic variation in ovine PRICAG3, and the variation found in this study could be functionally important for AMPK activity, which in turn may affect meat quality traits in sheep. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
WOS关键词	ACTIVATED PROTEIN-KINASE ; PIG SKELETAL-MUSCLE ; MEAT QUALITY ; LIPID-METABOLISM ; GLYCOGEN-CONTENT ; ASSOCIATION ; MUTATIONS ; BINDING ; ALLELE ; DNA
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000356980200018
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2555583
专题	寒区旱区环境与工程研究所
通讯作者	Wang, Ruoyu
作者单位	1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Fac Agr & Life Sci, Gene Marker Lab, Lincoln 7647, New Zealand
推荐引用方式 GB/T 7714	Yang, Guo,Zhou, Huitong,Wang, Ruoyu,et al. Variation in the ovine PRKAG3 gene[J]. GENE,2015,567(2):251-254.
APA	Yang, Guo,Zhou, Huitong,Wang, Ruoyu,&Hickford, Jon.(2015).Variation in the ovine PRKAG3 gene.GENE,567(2),251-254.
MLA	Yang, Guo,et al."Variation in the ovine PRKAG3 gene".GENE 567.2(2015):251-254.

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