Relationship of beta-glucuronidase to differentiation and invasion of human colorectal carcinoma

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	Relationship of beta-glucuronidase to differentiation and invasion of human colorectal carcinoma
	Feng, S; Song, JD; Feng, S, Acad Sinica, Inst Appl Ecol, Microbial Engn Dept, Shenyang 110015, Peoples R China
刊名	CHINESE MEDICAL JOURNAL
	1999
卷号	112 期号:9 页码:854-857
关键词	Beta-glucuronidase Colorectal Carcinoma Differentiation Invasion
ISSN号	0366-6999
英文摘要	Objective To investigate the relationship between beta-glucuronidase and tumor differentiation and invasion in human colorectal carcinoma. Methods The expression of beta-glucuronidase in human colorectal carcinoma tissue was determined by streptavidin-peroxidase; immunohistochemical method. Four human colorectal carcinoma cell lines were analysed for beta-glucuronidase in the medium by spectrophotometry method. Results Compared with well differentiated colorectal carcinomas, beta-glucuronidase expression was higher in poorly differentiated tumors. An association was found between beta-glucuronidase overexpression in colorectal carcinoma and more advanced tumor Dukes' stage (P < 0. 05). With increasing maximum depth of invasion, tumors more frequently show higher beta-glucuronidase expression (P < 0.05). The analysis of colorectal carcinoma cell lines for P-glucuronidase showed that low levels of beta-glucuronidase (activity range, 1.29 to 1.96 mu g/10(6) cells per hour) were associated with the well differentiated cell lines which have low invasiveness (CX1, CCL187), and this was in contrast to the elevated level of the enzyme (2.46 to 2.73 mu g/10(6) cells per hour) present in the medium derived from the poorly differentiated cells with high invasiveness (CCLI227, CCL228) ( P < 0.05). Conclusions Poorly differentiated human colorectal carcinoma cells could synthesize more beta-glucuronidase, enhance local invasion. Immunohistochemical staining for beta-glucuronidase might be helpful to diagnosing the differentiation degree and invasiveness of human colorectal carcinomas, and the secreted beta-glucuronidase might provide a useful measurement of the differentiation degree and invasiveness of cultured human colorectal carcinoma cells.
学科主题	Medicine, General & Internal
语种	英语
WOS记录号	WOS:000083134000025
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55601]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Feng, S, Acad Sinica, Inst Appl Ecol, Microbial Engn Dept, Shenyang 110015, Peoples R China
推荐引用方式 GB/T 7714	Feng, S,Song, JD,Feng, S, Acad Sinica, Inst Appl Ecol, Microbial Engn Dept, Shenyang 110015, Peoples R China. Relationship of beta-glucuronidase to differentiation and invasion of human colorectal carcinoma[J]. CHINESE MEDICAL JOURNAL,1999,112(9):854-857.
APA	Feng, S,Song, JD,&Feng, S, Acad Sinica, Inst Appl Ecol, Microbial Engn Dept, Shenyang 110015, Peoples R China.(1999).Relationship of beta-glucuronidase to differentiation and invasion of human colorectal carcinoma.CHINESE MEDICAL JOURNAL,112(9),854-857.
MLA	Feng, S,et al."Relationship of beta-glucuronidase to differentiation and invasion of human colorectal carcinoma".CHINESE MEDICAL JOURNAL 112.9(1999):854-857.