Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants

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	Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants
	Li, QR; Di Fiore, S; Fischer, R; Wang, M; Wang, M, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
刊名	BOTANICAL BULLETIN OF ACADEMIA SINICA
	2003
卷号	44 期号:3 页码:193-198
关键词	Chloroplast Nicotiana Tabacum Terpenoid Indole Alkaloids Tryptophan Decarboxylase
ISSN号	0006-8063
英文摘要	Tryptophan decarboxylase (TDC) is a key enzyme in the biosynthesis of terpenoid indole alkaloids (TIAs) from plants such as Catharanthus roseus. We transformed tobacco plants with tdc gene and expressed chloroplast-targeted versions of TDC protein. TDC was shown to be over-expressed in chloroplasts of tobacco plants by Western blot analysis and TDC enzymatic assay. The effective expression of TDC lead to accumulation of tryptamine in transgenic tobacco plants. T-1 plants showed higher levels of TDC expression than T-0 plants. In order to confirm that TDC was correctly targeted and expressed in chloroplasts, they were isolated from T-1 transgenic tobacco plants, and the different fractions of purified chloroplasts were analyzed. Transgenic plants displayed the phenotype characteristic of necrotic syndromes, which was related to the accumulation of tryptamine.
学科主题	Plant Sciences
语种	英语
WOS记录号	WOS:000184800100003
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55377]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Wang, M, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Li, QR,Di Fiore, S,Fischer, R,et al. Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants[J]. BOTANICAL BULLETIN OF ACADEMIA SINICA,2003,44(3):193-198.
APA	Li, QR,Di Fiore, S,Fischer, R,Wang, M,&Wang, M, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2003).Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants.BOTANICAL BULLETIN OF ACADEMIA SINICA,44(3),193-198.
MLA	Li, QR,et al."Expression of tryptophan decarboxylase in chloroplasts of transgenic tobacco plants".BOTANICAL BULLETIN OF ACADEMIA SINICA 44.3(2003):193-198.