Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme

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	Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme
	Hudek, L; Rai, LC; Freestone, D; Michalczyk, A; Gibson, M; Song, YF; Ackland, ML; Ackland, ML, Deakin Univ, Ctr Cellular & Mol Biol, Sch Life & Environm Sci, Burwood Campus,221 Burwood Highway, Burwood, Vic 3125, Australia
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2009
卷号	75 期号:3 页码:784-791
ISSN号	0099-2240
英文摘要	Zinc homeostasis was investigated in Nostoc punctiforme. Cell tolerance to Zn2+ over 14 days showed that ZnCl2 levels above 22 mu M significantly reduced cell viability. After 3 days in 22 mu M ZnCl2, ca. 12% of the Zn2+ was in an EDTA-resistant component, suggesting an intracellular localization. Zinquin fluorescence was detected within cells exposed to concentrations up to 37 mu M relative to 0 mu M treatment. Radiolabeled Zn-65 showed Zn2+ uptake increased over a 3-day period, while efflux occurred more rapidly within a 3-h time period. Four putative genes involved in Zn2+ uptake and efflux in N. punctiforme were identified: (i) the predicted Co/Zn/Cd cation transporter, putative CDF; (ii) the predicted divalent heavy-metal cation transporter, putative Zip; (iii) the ATPase component and Fe/Zn uptake regulation protein, putative Fur; and (iv) an ABC-type Mn/Zn transport system, putative zinc ZnuC, ZnuABC system component. Quantitative real-time PCR indicated the responsiveness of all four genes to 22 mu M ZnCl2 within 3 h, followed by a reduction to below basal levels after 24 h by putative ZIP, ZnuC, and Fur and a reduction to below basal level after 72 h by putative CDF efflux gene. These results demonstrate differential regulation of zinc transporters over time, indicating a role for them in zinc homeostasis in N. punctiforme.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000262690100029
资助机构	International Science Linkage Programme ; Australia-China Special Fund for Scientific and Technological Cooperation.
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54261]
专题	沈阳应用生态研究所_沈阳应用生态研究所
通讯作者	Ackland, ML, Deakin Univ, Ctr Cellular & Mol Biol, Sch Life & Environm Sci, Burwood Campus,221 Burwood Highway, Burwood, Vic 3125, Australia
推荐引用方式 GB/T 7714	Hudek, L,Rai, LC,Freestone, D,et al. Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(3):784-791.
APA	Hudek, L.,Rai, LC.,Freestone, D.,Michalczyk, A.,Gibson, M.,...&Ackland, ML, Deakin Univ, Ctr Cellular & Mol Biol, Sch Life & Environm Sci, Burwood Campus,221 Burwood Highway, Burwood, Vic 3125, Australia.(2009).Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(3),784-791.
MLA	Hudek, L,et al."Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.3(2009):784-791.