Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis

doi:10.1016/j.ecoenv.2018.12.046

	Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis
	Wang, Yingnan 2,3; Peng, Xiaoyuan 2; Salvato, Fernanda 4; Wang, Yongcui 1; Yan, Xiufeng 2; Zhou, Zhiqiang 3; Lin, Jixiang 2,4
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2019-04-30
卷号	171 页码:12-25
关键词	Cotyledon Early seedling, Proteomics Ricinus communis Salinity
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2018.12.046
英文摘要	Soil salinity is a major abiotic stress affecting crop growth and productivity. Ricinus communis has good salt tolerance and is also an important oilseed crop throughout the world. Early seedling stage (such as cotyledon expansion stage) is the most vulnerable period for plant under stresses. However, little information exist concerning the physiological and molecular mechanisms of Ricinus communis seedlings and the role play by cotyledons and true leaf under salt stress. In the present study, biomass, photosynthesis, chlorophyll fluorescence, inorganic ions and organic solutes contents were measured, and two dimensional gel electrophoresis-based proteomic technology was employed to identify the differentially abundant proteins in the salt-treated Ricinus communis cotyledons and true leaves. The results showed that salt stress reduced growth and photosynthesis in the seedlings. With increasing salinity, the Na+ content increased and K+ content decreased in both cotyledons and leaves, but the true leaves had lower Na+ and higher K+ contents. Soluble sugars and proline are the primary organic solutes to cope with osmotic stress. In addition, proteomic analysis revealed 30 and 42 differentially accumulated protein spots in castor cotyledon and true leaf under salt stress, respectively. Most of the identified proteins were involved in carbohydrate and energy metabolism, photosynthesis, genetic information process, reactive oxygen species metabolism, amino acid metabolism and cell structure. The physiological and proteomic results highlighted that cotyledons accumulated a large number of Na+ and provided more energy to help true leaves cope with salt stress. The true leaves saved carbon structures to synthesize osmotic substances, and the enhancement of chlorophyll synthesis and electron transfer in true leaves could also maintain photosynthesis under salt stress. These findings provide new insights into different physiological mechanisms in cotyledon and true leaf of Ricinus communis response to salt stress during early seedling stage.
资助项目	Fundamental Research Funds for the Central Universities[2572018BS02] ; National Natural Science Foundation of China[41601588]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000459217600002
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/123931]
专题	中国科学院沈阳应用生态研究所
通讯作者	Lin, Jixiang
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China 2.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat, Harbin 150040, Heilongjiang, Peoples R China 3.Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Heilongjiang, Peoples R China 4.North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA
推荐引用方式 GB/T 7714	Wang, Yingnan,Peng, Xiaoyuan,Salvato, Fernanda,et al. Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,171:12-25.
APA	Wang, Yingnan.,Peng, Xiaoyuan.,Salvato, Fernanda.,Wang, Yongcui.,Yan, Xiufeng.,...&Lin, Jixiang.(2019).Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,171,12-25.
MLA	Wang, Yingnan,et al."Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 171(2019):12-25.