Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes

doi:10.1093/femsec/fiv151

	Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes
	Jiang, Yong 2; Li, Hui 2; Li, Xiaobin 1,2; Wang, Yafei 2; Brown, Celeste J.3; Yao, Fei 1,2; Top, Eva M.3
刊名	FEMS MICROBIOLOGY ECOLOGY
	2016
卷号	92 期号:1
关键词	Incp-1 Epsilon Plasmid Comparative Genome Analysis Antibiotic Resistance Horizontal Gene Transfer Petroleum Polluted Soil Wastewater
ISSN号	0168-6496
DOI	10.1093/femsec/fiv151
英文摘要	The IncP-1 epsilon subgroup is a recently identified phylogenetic clade within IncP-1 plasmids, which plays an important role in the spread of antibiotic resistance and degradation of xenobiotic pollutants. Here, four IncP-1 epsilon plasmids were exogenously captured from a petroleum-contaminated habitat in China and compared phylogenetically and genomically with previously reported IncP-1 epsilon and other IncP-1 plasmids. The IncP-1 epsilon plasmids can be clearly subdivided into two subclades, designated as epsilon-I and epsilon-II, based on phylogenetic analysis of backbone proteins TraI and TrfA. This was further supported by comparison of concatenated backbone genes. Moreover, the two subclades differed in the transposon types, phenotypes and insertion locations of the accessory elements. The accessory genes on epsilon-I plasmids were inserted between parA and traC, and harbored ISPa17 and Tn402-like transposon modules, typically carrying antibiotic resistance genes. In contrast, the accessory elements on epsilon-II plasmids were typically located between trfA and oriV, and contained IS1071, which was commonly inserted within the Tn501-like transposon, typically harboring a cluster of genes encoding mercury resistance and/or catabolic pathways. Our study is one of the first to compare IncP-1 plasmid genomes from China, expands the available collection of IncP-1 epsilon plasmids and enhances our understanding of their diversity, biogeography and evolutionary history.
WOS研究方向	Microbiology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000371249300008
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122501]
专题	中国科学院沈阳应用生态研究所
通讯作者	Li, Hui
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Wenhua Rd 72, Shenyang 110164, Peoples R China 3.Univ Idaho, Inst Bioinformat & Evolutionary Studies IBEST, Dept Biol Sci, Moscow, ID 83844 USA
推荐引用方式 GB/T 7714	Jiang, Yong,Li, Hui,Li, Xiaobin,et al. Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes[J]. FEMS MICROBIOLOGY ECOLOGY,2016,92(1).
APA	Jiang, Yong.,Li, Hui.,Li, Xiaobin.,Wang, Yafei.,Brown, Celeste J..,...&Top, Eva M..(2016).Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes.FEMS MICROBIOLOGY ECOLOGY,92(1).
MLA	Jiang, Yong,et al."Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes".FEMS MICROBIOLOGY ECOLOGY 92.1(2016).