Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway

doi:10.1016/j.aquatox.2015.08.004

	Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway
	Chen, Jianjie 1; Cao, Jinling 1; Xie, Lingtian 2; Wang, Jundong 1; Feng, Cuiping 1; Song, Jing 1
刊名	AQUATIC TOXICOLOGY
	2015-10-01
卷号	167 页码:180-190
关键词	Sesamin Fluoride Kidney Oxidative Stress Apoptosis Jnk
ISSN号	0166-445X
DOI	10.1016/j.aquatox.2015.08.004
英文摘要	Sesamin, a major lignan derived from sesame seeds, has been reported to have many benefits and medicinal properties. However, its protective effects against fluoride-induced injury in kidney of fish have not been clarified. Previously we found that fluoride exposure caused damage and apoptosis in the kidneys of the common carp, Cyprinus carpio. In this study, the effects of sesamin on renal oxidative stress and apoptosis in fluoride-exposed fish were determined. The results showed that sesamin alleviated significantly fluoride-induced renal damage and apoptosis of carp in a dose-dependent manner, indicated by the histopathological examination and ultrastructural observation. Moreover, treatment with sesamin also inhibited significantly fluoride-induced remarkable enhancement of reactive oxygen species (ROS) production and oxidative stress, such as the increase of lipid peroxidation level and the depletion of intracellular reduced glutathione (GSH) level in kidney. To explore the underlying mechanisms of sesamin action, we found that activities of caspase-3 were notably inhibited by treatment with sesamin in the kidney of fluoride-exposed fish. Sesamin decreased the levels of p-JNK protein in kidney, which in turn inactivated pro-apoptotic signaling events by restoring the balance between mitochondrial pro- and anti-apoptotic Bcl-2 and Bax proteins and by decreasing the release of mitochondrial cytochrome c in kidney of fluoride-exposed fish. JNK was also involved in the mitochondrial extrinsic apoptotic pathways of sesamin effects against fluoride-induced renal injury by regulating the levels of p-c-Jun, necrosis factor-alpha (TNF-alpha) and Bak proteins. These findings indicated that sesamin could protect kidney against fluoride-induced apoptosis by the oxidative stress downstream-mediated change in the inactivation of JNK signaling pathway. Taken together, sesamin plays an important role in maintaining renal health and preventing kidney from toxic damage induced by fluoride. (C) 2015 Elsevier B.V. All rights reserved.
WOS研究方向	Marine & Freshwater Biology ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000362859400017
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/122231]
专题	中国科学院沈阳应用生态研究所
通讯作者	Wang, Jundong
作者单位	1.Shanxi Agr Univ, State Key Lab Ecol Anim Husb & Environm Vet Med, Taigu 030801, Shanxi, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jianjie,Cao, Jinling,Xie, Lingtian,et al. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway[J]. AQUATIC TOXICOLOGY,2015,167:180-190.
APA	Chen, Jianjie,Cao, Jinling,Xie, Lingtian,Wang, Jundong,Feng, Cuiping,&Song, Jing.(2015).Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway.AQUATIC TOXICOLOGY,167,180-190.
MLA	Chen, Jianjie,et al."Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway".AQUATIC TOXICOLOGY 167(2015):180-190.