Characterization and quantification of the triterpenoids in different parts of Xanthoceras sorbifolia by HPLC-ESI-MS

doi:10.1016/j.jpba.2011.01.030

	Characterization and quantification of the triterpenoids in different parts of Xanthoceras sorbifolia by HPLC-ESI-MS
	Ling, Junhong 1; Liu, Lili 1; Wang, Yixiang 1; Li, Zeyun 1; Liu, Ran 1; Li, Qing 1; Wang, Yi 2; Yang, Baizhen 2; Chen, Xiaohui 1; Bi, Kaishun 1
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2011-05-15
卷号	55 期号:2 页码:259-264
关键词	Xanthoceras Sorbifolia Sorbifoside Xanthoceraside Hplc-esi-ms Quantification Alzheimer's Disease
ISSN号	0731-7085
DOI	10.1016/j.jpba.2011.01.030
英文摘要	Two new triterpenoid glycosides, sorbifoside A (2) and B (3), were isolated from the husks of Xanthoceras sorbifolia along with two known saponins (1, 5). Their structures were established on the basis of 1D and 2D NMR data. A simple and sensitive assay was developed for the simultaneous determination of 6 triterpenoids (1-6, including two triterpenoid aglycones) in X. sorbifolia based on high performance liquid chromatography-mass spectrometry ( HPLC-MS) coupled to an electrospray ionization (ESI) interface. The analytes were detected by positive ESI ionization mode and quantified by selected ion monitoring (SIM). All the linear regressions were acquired with r(2) > 0.998. The precisions were evaluated by intra- and inter-day tests, and the relative standard deviation (RSD) values were within the range of 2.0-2.8% and 1.7-2.9%, respectively. The recoveries for the quantified compounds were observed over the range of 95.3-104.7% with RSD values less than 4.6%. The method developed was successfully applied for simultaneous quantification of the six triterpenoids in X. sorbifolia, and our results showed that the contents of triterpenoids in different parts of X. sorbifolia varied significantly. (C) 2011 Elsevier B.V. All rights reserved.
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000288523300004
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/121755]
专题	中国科学院沈阳应用生态研究所
通讯作者	Bi, Kaishun
作者单位	1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Ling, Junhong,Liu, Lili,Wang, Yixiang,et al. Characterization and quantification of the triterpenoids in different parts of Xanthoceras sorbifolia by HPLC-ESI-MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,55(2):259-264.
APA	Ling, Junhong.,Liu, Lili.,Wang, Yixiang.,Li, Zeyun.,Liu, Ran.,...&Bi, Kaishun.(2011).Characterization and quantification of the triterpenoids in different parts of Xanthoceras sorbifolia by HPLC-ESI-MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,55(2),259-264.
MLA	Ling, Junhong,et al."Characterization and quantification of the triterpenoids in different parts of Xanthoceras sorbifolia by HPLC-ESI-MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 55.2(2011):259-264.