Effects of methamidophos on the community structure, antagonism towards Rhizoctonia solani, and phlD diversity of soil Pseudomonas

doi:10.1080/03601231003613609

	Effects of methamidophos on the community structure, antagonism towards Rhizoctonia solani, and phlD diversity of soil Pseudomonas
	Wu, Minna 2,3; Li, Xinyu ; Zhang, Huiwen 1; Cai, Yinghui 3; Zhang, Chenggang
刊名	JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
	2010
卷号	45 期号:3 页码:222-228
关键词	Methamidophos Pseudomonas Soil Microbial Community Antagonism Phld Amplified Ribosomal Dna Restriction Analysis (Ardra)
ISSN号	0360-1234
DOI	10.1080/03601231003613609
英文摘要	A microcosm incubation study using an aquic brown soil from northeast China (a Cambisol in the UN Food and Agriculture Organization FAO Soil Taxonomy) was conducted to examine the effects of different concentrations (0, 50, 150, and 250 mg kg-1) of methamidophos (O,S-dimethyl phosphoramidothioato) on Pseudomonas, one of the most important gram-negative bacteria in soil. Amplified ribosomal DNA restriction analysis (ARDRA) was performed to study the Pseudomonas community structure, an in vitro assay was made to test the antagonistic activity of isolated Pseudomonas strains against soil-borne Rhizoctonia solani, a major member of the pathogens highly related to soil-borne plant diseases, and special primer amplification and sequencing were performed to investigate the diversity of phlD, an essential gene in the biosynthesis of 2, 4-diacetylphloroglucinol (2, 4-DAPG), which has biocontrol activity in phlD+isolates. With exposure to increasing methamidophos concentrations, the total number of soil Pseudomonas ARDRA patterns decreased significantly, but with less change in the same treatments over 1, 3, and 5 weeks of incubation. The number of isolated Pseudomonas strains with antagonistic activity against R. solani as well as the diversity and appearance frequency of the strains' phlD gene also decreased with increasing concentrations of methamidophos, especially at high methamidophos concentrations. Applying methamidophos could increase the risk of soil-borne plant diseases by decreasing the diversity of the soil Pseudomonas community and the amount of R. solani antagonists, particularly those with the phlD gene.
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000277586300006
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/121433]
专题	中国科学院沈阳应用生态研究所
通讯作者	Zhang, Huiwen
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Grp Microbiol Resource, Shenyang, Peoples R China 2.Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Minna,Li, Xinyu,Zhang, Huiwen,et al. Effects of methamidophos on the community structure, antagonism towards Rhizoctonia solani, and phlD diversity of soil Pseudomonas[J]. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES,2010,45(3):222-228.
APA	Wu, Minna,Li, Xinyu,Zhang, Huiwen,Cai, Yinghui,&Zhang, Chenggang.(2010).Effects of methamidophos on the community structure, antagonism towards Rhizoctonia solani, and phlD diversity of soil Pseudomonas.JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES,45(3),222-228.
MLA	Wu, Minna,et al."Effects of methamidophos on the community structure, antagonism towards Rhizoctonia solani, and phlD diversity of soil Pseudomonas".JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES 45.3(2010):222-228.