Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri
	Wang, M. Q.1,3; Wang, B. J.1; Liu, M.1; Jiang, K. Y.1; Wang, L.1,2,4
刊名	ISJ-INVERTEBRATE SURVIVAL JOURNAL
	2018
卷号	15 页码:294-301
关键词	Apoptosis Chlamys farreri Innate immunity
ISSN号	1824-307X
通讯作者	Wang, L.(wanglei@qdio.ac.cn)
英文摘要	The defender against apoptotic cell death 1 (DAD1) was a negative regulator to inhibit the apoptosis process. In the present study, a DAD1 gene (designated as CfDAD1) was identified in Zhikong scallop Chlamys farreri. Its full-length cDNA sequence of CfDAD1 contained a 342 bp open reading frame (ORF), which encoded a mature protein of 113 amino acids. A DAD domain was revealed from the deduced protein sequence of CfDAD1. The mRNA transcripts of CfDAD1 could be detectable in all the investigated tissues, including hemocytes, muscle, mantle, gill, hepatopancreas and gonad, among which the maximum expression level were found in hemocytes. Being infected with Vibrio splendidus, Staphylococcus aureus or Yarrowia lipolytica, the mRNA expression levels of CfDAD1 in hemocytes were all significantly up-regulated. Moreover, the apoptosis level of CfDAD1-suppressed group was significant higher than those of control groups. All these results indicated that CfDAD1 was efficient negative regulator of apoptosis and involved in the innate immune responses of scallop.
资助项目	National Natural Science Foundation of China[U1706209] ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ07] ; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106] ; STS Regional Centre Project (Fujian province) from Chinese Academy of Sciences
WOS研究方向	Immunology ; Zoology
语种	英语
出版者	INVERTEBRATE SURVIVAL JOURNAL
WOS记录号	WOS:000443548300005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/160027]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266400, Peoples R China
推荐引用方式 GB/T 7714	Wang, M. Q.,Wang, B. J.,Liu, M.,et al. Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:294-301.
APA	Wang, M. Q.,Wang, B. J.,Liu, M.,Jiang, K. Y.,&Wang, L..(2018).Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,294-301.
MLA	Wang, M. Q.,et al."Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):294-301.