Growth Performance, Digestive Enzymes, and TOR Signaling Pathway of Litopenaeus vannamei Are Not Significantly Affected by Dietary Protein Hydrolysates in Practical Conditions

doi:10.3389/fphys.2018.00998

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Growth Performance, Digestive Enzymes, and TOR Signaling Pathway of Litopenaeus vannamei Are Not Significantly Affected by Dietary Protein Hydrolysates in Practical Conditions
	Shao, Jianchun 1,2,3; Zhao, Wei 4; Liu, Xinwei 1,2,3; Wang, Lei 1,2
刊名	FRONTIERS IN PHYSIOLOGY
	2018-08-14
卷号	9 页码:8
关键词	Litopenaeus vannamei protein hydrolysates growth digestive enzymes TOR signaling pathway
ISSN号	1664-042X
DOI	10.3389/fphys.2018.00998
通讯作者	Wang, Lei(wanglei@qdio.ac.cn)
英文摘要	Protein hydrolysates have been widely reported as the protein source of aquatic feed. However, previous studies on protein hydrolysates focused on fish under experimental conditions. In this study, a 6-week feeding trial in a greenhouse was conducted to investigate the effects of partially replacing fishmeal by protein hydrolysates on growth performance, digestive enzymes, and TOR signaling pathway of Litopenaeus vannamei under practical conditions. This involved randomly selecting 72,000 shrimps (initial body weight 2.26 +/- 0.02 g) and placing them in groups inside nine concrete tanks (L 5 m x W 5 m x H 1 m) (3 treatments x 3 replicates x 8000 individuals per concrete tank). Two isonitrogenous (430 g kg(-1)) and isolipidic (80 g kg(-1)) diets were prepared: fishmeal diet (FM) containing 400g kg(-1) fishmeal, and protein hydrolysates diet (PH) in which 15% of the fishmeal was replaced by protein hydrolysates. A commercial diet (CD) was used as reference. The final weight (FW), percent weight gain (PWG), specific growth ratio (SGR), and total weight for each tank (TW) of L. vannamei fed with FM and PH diets were not significantly different (P > 0.05). However, shrimp fed with PH diet had significantly higher FW, PWG, SGR, and TW values than those fed with CD diet (P < 0.05). Trypsin activity was significantly lower in shrimp fed with CD diet, than in shrimp fed with FM and PH diets (P < 0.05). However, trypsin activity of L. vannamei fed with FM and PH diets were not significantly different (P > 0.05). The mRNA expression of tor, s6k, and 4e-bp genes were not significantly affected between FM and PH diets, while tor and s6k expression levels of CD diet were significantly down-regulated. Based on the above data, 15% replacement of fishmeal with protein hydrolysates did not make any difference on shrimps compared with FM group. Therefore, protein hydrolysates can partially replace fishmeal as the protein source of shrimp formula feed in practical conditions.
资助项目	National Science Foundation of China[41406151] ; STS fund of Chinese Academy of Sciences (Fujian)
WOS研究方向	Physiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000441680900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/159867]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Lei
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Jinan Univ, Res Ctr Hydrobiol, Dept Life Sci & Biotechnol, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Shao, Jianchun,Zhao, Wei,Liu, Xinwei,et al. Growth Performance, Digestive Enzymes, and TOR Signaling Pathway of Litopenaeus vannamei Are Not Significantly Affected by Dietary Protein Hydrolysates in Practical Conditions[J]. FRONTIERS IN PHYSIOLOGY,2018,9:8.
APA	Shao, Jianchun,Zhao, Wei,Liu, Xinwei,&Wang, Lei.(2018).Growth Performance, Digestive Enzymes, and TOR Signaling Pathway of Litopenaeus vannamei Are Not Significantly Affected by Dietary Protein Hydrolysates in Practical Conditions.FRONTIERS IN PHYSIOLOGY,9,8.
MLA	Shao, Jianchun,et al."Growth Performance, Digestive Enzymes, and TOR Signaling Pathway of Litopenaeus vannamei Are Not Significantly Affected by Dietary Protein Hydrolysates in Practical Conditions".FRONTIERS IN PHYSIOLOGY 9(2018):8.