Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis

doi:10.3390/md16030085

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	Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis
	Cao, Ruobing 1,2,3; Jin, Weihua 4; Shan, Yeqi 1,2,3; Wang, Ju 1,2,3; Liu, Ge 1,2,3; Kuang, Shan 1,2; Sun, Chaomin 1,2
刊名	MARINE DRUGS
	2018-03-01
卷号	16 期号:3 页码:18
关键词	polysaccharide cancer filiform structures beta III-tubulin anoikis adhesion
ISSN号	1660-3397
DOI	10.3390/md16030085
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要	Tumor cells that acquire metastatic potential have developed resistance to anoikis, a cell death process, after detachment from their primary site to the second organ. In this study, we investigated the molecular mechanisms of a novel marine bacterial polysaccharide EPS11 which exerts its cytotoxic effects through affecting cancer cell adhesion and anoikis. Firstly, we found that EPS11 could significantly affect cell proliferation and block cell adhesion in A549 cells. We further demonstrated that the expression of several cell adhesion associated proteins is downregulated and the filiform structures of cancer cells are destroyed after EPS11 treatment. Interestingly, the destruction of filiform structures in A549 cells by EPS11 is in a dose-dependent manner, and the inhibitory tendency is very consistent with that observed in the cell adhesion assay, which confirms that filiform structures play important roles in modulating cell adhesion. Moreover, we showed that EPS11 induces apoptosis of A549 cells through stimulating beta III-tubulin associated anoikis: (i) EPS11 inhibits the expression of beta III-tubulin in both transcription and translation levels; and (ii) EPS11 treatment dramatically decreases the phosphorylation of protein kinase B (PKB or AKT), a critical downstream effector of beta III-tubulin. Importantly, EPS11 evidently inhibits the growth of A549-derived tumor xenografts in vivo. Thus, our results suggest that EPS11 may be a potential candidate for human non-small cell lung carcinoma treatment via blocking filiform structure mediated adhesion and stimulating beta III-tubulin associated anoikis.
资助项目	Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Young Scholar Program of Shandong Province ; 100-Talent Project of Chinese Academy of Sciences
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000428510200011
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/159205]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China 4.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Cao, Ruobing,Jin, Weihua,Shan, Yeqi,et al. Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis[J]. MARINE DRUGS,2018,16(3):18.
APA	Cao, Ruobing.,Jin, Weihua.,Shan, Yeqi.,Wang, Ju.,Liu, Ge.,...&Sun, Chaomin.(2018).Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis.MARINE DRUGS,16(3),18.
MLA	Cao, Ruobing,et al."Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis".MARINE DRUGS 16.3(2018):18.