A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp

doi:10.1210/en.2017-03253

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp
	Guo, Qing 1,2,3; Li, Shihao 1,2; Lv, Xinjia1,3 ; Xiang, Jianhai1,2 ; Sagi, Amir 4; Manor, Rivka 4; Li, Fuhua 1,2
刊名	ENDOCRINOLOGY
	2018-05-01
卷号	159 期号:5 页码:2173-2185
ISSN号	0013-7227
DOI	10.1210/en.2017-03253
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	The insulin-like androgenic gland hormone (IAG) is the key regulator in crustacean male sexual differentiation. As a secreted peptide hormone, IAG might perform its biological function through interacting with the membrane receptor. However, the receptor of IAG remains unclear. In the current study, a putative IAG receptor gene (FcIAGR) was identified in Fenneropenaeus chinensis. The deduced amino acid sequence of FcIAGR contained several conserved domains of insulin-like receptor proteins, including two L domains (L1 and L2), a cysteine-rich domain, three fibronectin III domains, a transmembrane domain, and an intracellular tyrosine kinase domain. Tissue distribution and in situ hybridization analysis showed that FcIAGR was predominantly expressed in the androgenic gland and testis in male F. chinensis. Protein colocalization analysis in HEK293 cells showed that FcIAGR could colocalize with both FcIAG1 and FcIAG2, respectively. Yeast two-hybrid assay further confirmed the interactions between FcIAGR and FcIAGs. After a long-term silencing of FcIAGR with double-stranded RNA, most of the germ cells in the testis were arrested at the secondary spermatocytes, whereas those in the control developed into sperm cells. The data indicated that FcIAGR was the receptor of FcIAGs in F. chinensis. The current study provides insight into the mechanism that the insulin-like signaling pathway regulates the male sexual differentiation in Decapoda crustaceans.
资助项目	National Natural Science Foundation of China[31461143007] ; National Natural Science Foundation of China[31302171] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000432685100024
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/159183]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
推荐引用方式 GB/T 7714	Guo, Qing,Li, Shihao,Lv, Xinjia,et al. A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp[J]. ENDOCRINOLOGY,2018,159(5):2173-2185.
APA	Guo, Qing.,Li, Shihao.,Lv, Xinjia.,Xiang, Jianhai.,Sagi, Amir.,...&Li, Fuhua.(2018).A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp.ENDOCRINOLOGY,159(5),2173-2185.
MLA	Guo, Qing,et al."A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp".ENDOCRINOLOGY 159.5(2018):2173-2185.