Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)
	Liu, X.1,2,3; Wang, M.1,2; Shao, J.1,2,3; Wang, B.1,2; Jiang, K.1,2; Liu, M.1,2; Wang, L.1,2
刊名	ISJ-INVERTEBRATE SURVIVAL JOURNAL
	2018
卷号	15 页码:52-60
关键词	Litopenaeus vannamei molecular cloning mTOR Rheb
ISSN号	1824-307X
通讯作者	Liu, M.(liumei@qdio.ac.cn) ; Wang, L.(wanglei@qdio.ac.cn)
英文摘要	The Ras family GTPase Rheb, a GTP-binding protein, binds specifically to the mTOR catalytic domain and induces activation of the mTOR catalytic function. Furthermore, Rheb is related to a stable modification of the configuration of mTORC1 that increases access of substrates to their binding site on the raptor polypeptide. In the present research, a cDNA of 898 bp for the Litopenaeus vannamei Rheb was cloned via rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of Rheb contained an open reading frame (ORF) of 549 bp, which encoded a protein of 182 amino acids. The amino acid sequence of Rheb shared more than 60% similarity with other identified Rheb proteins. A Ras domain (from P3 to A169) was found in the amino acid sequence of Rheb that can react selectively and non-covalently with GTP. The mRNA transcripts of Rheb were consistently expressed in all the tested tissues, including muscle, gill, hepatopancreas, eyestalk, intestine and stomach. The mRNA expression profiles of Rheb in muscle after the stimulation with rapamycin were promoted, which further proved that Rheb protein could be a feedback regulator to mTOR signaling pathway. Furthermore, the results of the present study indicated that Rheb played an important role in the regulation of mTOR signaling pathway during the stimulation of dietary restriction, amino acid supplementation and rapamycin stimulation in shrimp.
资助项目	National Natural Science Foundation of China[41406151] ; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106]
WOS研究方向	Immunology ; Zoology
语种	英语
出版者	INVERTEBRATE SURVIVAL JOURNAL
WOS记录号	WOS:000428589300002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158459]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, M.; Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, X.,Wang, M.,Shao, J.,et al. Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:52-60.
APA	Liu, X..,Wang, M..,Shao, J..,Wang, B..,Jiang, K..,...&Wang, L..(2018).Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei).ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,52-60.
MLA	Liu, X.,et al."Molecular cloning and characterization of Rheb from white shrimp (Litopenaeus vannamei)".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):52-60.