Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis

doi:10.1016/j.fsi.2017.12.056

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis
	Jia, Zhihao 1,4; Wang, Mengqiang1 ; Zhang, Huan 1; Wang, Xiudan1,4 ; Lv, Zhao 1,4; Wang, Lingling 2; Song, Linsheng 2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018-03-01
卷号	74 页码:332-340
关键词	Eriocheir sinensis Serine proteinase Clip domain Innate immunity proPO Microbial binding
ISSN号	1050-4648
DOI	10.1016/j.fsi.2017.12.056
通讯作者	Song, Linsheng(lshsong@qdio.ac.cn)
英文摘要	Clip-domain serine proteinase is an important serine proteinase family involved in many biological processes, which is only found in invertebrates. In the present study, the full-length cDNA of a clip domain serine proteinase (designed as EsCDSP) gene was cloned from Chinese mitten crab Eriocheir sinensis using rapid amplification of cDNA ends (RACE) technique. It was of 1488 bp with an open reading frame (ORF) of 1134 bp encoding a polypeptide of 377 amino acids. There were a signal peptide, a clip domain, and a Tryp_SPc domain in the deduced amino acid sequence of EsCDSP. Highly conserved cysteine residues were identified in the clip domain and Tryp_SPc domain. EsCDSP shared similarities of 40%-61% with CDSPs from Penaeus monodon (ACP19562.1), Scylla paramamosain (CCW43200.1), Drosophila melanogaster (NP_649734.2) and Delia antiqua (AAW57295.1). It was clustered with other CDSPs from crabs in the phylogenetic tree. EsCDSP transcript was highly expressed in hemocytes and it could response to the stimulations of Vibro anguillarum and Pichia pastoris. rEsCDSP could activate proPO system and significantly increase the PO activity of HLS. In addition, rEsCDSP could bond to Aeromonas hydrophila, Vibro anguillarum and Vibro alginolyticus, and reduced the mortality rate causing by pathogen infection. All the results suggested that EsCDSP was an important immune response participator involved in activation of the proPO system of crab.
资助项目	National Science Foundation of China[31530069] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian high level talent innovation support program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; AoShan Talents Cultivation Program
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000427344200035
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158376]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jia, Zhihao,Wang, Mengqiang,Zhang, Huan,et al. Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:332-340.
APA	Jia, Zhihao.,Wang, Mengqiang.,Zhang, Huan.,Wang, Xiudan.,Lv, Zhao.,...&Song, Linsheng.(2018).Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,74,332-340.
MLA	Jia, Zhihao,et al."Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 74(2018):332-340.