Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus

doi:10.1007/s10695-017-0445-1

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus
	Fu, Yuanshuai 1; Gao, Lina 1; Shi, Zhiyi1 ; You, Feng 2; Zhang, Junling 1; Li, Wenjuan 1
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2018-04-01
卷号	44 期号:2 页码:451-463
关键词	P. olivaceus lin-28a let-7 miRNAs Embryonic development Metamorphosis
ISSN号	0920-1742
DOI	10.1007/s10695-017-0445-1
通讯作者	Shi, Zhiyi(zyshi@shou.edu.cn)
英文摘要	Heterochronic lin-28 is a conserved RNA-binding protein that plays a key role in the timing of developmental events in organisms. As a crucial heterochronic gene, the protein controls developmental events of the second of four larval stages in Caenorhabditi elegans. Heterochronic let-7 miRNAs are often present in various species and highly conserved in sequence and biological function and are required for various biological processes. Previous studies showed that ten let-7 miRNAs were identified in the Japanese flounder (Paralichthys olivaceus) and that they were primarily expressed during metamorphosis. In this study, we clone and characterize the lin-28a gene from P. olivaceus and exhibit its dynamic expression pattern at different developmental stages and various adult tissues. The results show that the P. olivaceus lin-28a gene has high sequence similarity with other species and is highly expressed in the embryonic stage but weakly expressed in the larval stage. In addition, lin-28a overexpression causes cell proliferation and significantly promotes the levels of pre-let-7a and pre-let-7d while markedly depressing let-7a and let-7d expression in FEC (Flounder Embryonic Cell), which indicate that lin-28 possibly blocks the maturation of let-7 miRNAs. Additionally, lin-28a is identified as a target gene of let-7 miRNAs, and let-7 miRNAs directly regulate lin-28a expression by targeting its 3' UTR. Taken together, lin-28a along with let-7 miRNA participates in a lin-28/let-7 axis pathway that regulates cell division and timing of embryonic and metamorphic events in P. olivaceus.
资助项目	Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2015No01] ; National Natural Science Foundation of China[41506159] ; National Natural Science Foundation of China[41306128] ; Natural Science Foundation of Shanghai[15ZR1420600] ; Innovation Programme of Shanghai Municipal Education Commission[15ZZ083] ; Science and Technology Development Foundation of Shanghai Ocean University[A2-0203-00-100204]
WOS研究方向	Biochemistry & Molecular Biology ; Fisheries ; Physiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000428055300003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158357]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Shi, Zhiyi
作者单位	1.Shanghai Ocean Univ, Minist Agr, Key Lab Genet Resources Freshwater Aquaculture &, 999 Hu Cheng Huan Rd, Shanghai 201306, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Fu, Yuanshuai,Gao, Lina,Shi, Zhiyi,et al. Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(2):451-463.
APA	Fu, Yuanshuai,Gao, Lina,Shi, Zhiyi,You, Feng,Zhang, Junling,&Li, Wenjuan.(2018).Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus.FISH PHYSIOLOGY AND BIOCHEMISTRY,44(2),451-463.
MLA	Fu, Yuanshuai,et al."Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus".FISH PHYSIOLOGY AND BIOCHEMISTRY 44.2(2018):451-463.