The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities

doi:10.1016/j.ijbiomac.2017.12.009

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	The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities
	Jin, Weihua 1,2; Liu, Bing 1; Li, Shuai 1; Chen, Jing 1; Tang, Hong 1; Jiang, Di 1; Zhang, Quanbin 2; Zhong, Weihong 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-03-01
卷号	108 页码:307-313
关键词	Heteropolysaccharide Structural analysis Sargassum thunbergii
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2017.12.009
通讯作者	Zhang, Quanbin(qbzhang@qdio.ac.cn) ; Zhong, Weihong(whzhong@zjut.edu.cn)
英文摘要	Polysaccharide (ST) was prepared from Sargassum thunbergii using hot water. Two fractions (ST-1 and ST-2) were prepared using anion exchange chromatography. One desulfated polysaccharide (ST-1-DS) was also prepared. Electrospray ionization mass spectrometry (ESI MS) performed on ST-1-DS showed that the desulfated polysaccharides contained methyl glycosides of mono-sulfated and di-sulfated galactofucooligosaccharides. This result suggested that ST-1 might contain sulfated galactofucan, which consists of a backbone of alternating (Gal) and (Fuc)(n) and sulfated randomly on Gal and mainly on C-2 in Fuc. In addition, ST-1 was degraded in 1 M sulfuric acid. The solution was centrifuged, and the supernatant was concentrated and precipitated in ethanol to obtain the precipitate (ST-1-P). ST-1-P was then separated using gel chromatography and anion exchange chromatography to obtain the oligomers. ESI MS spectra of oligomers indicated that ST-1 mostly contained sulfated glucuronomannan and fucoglucuronan. ESI MS with collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) suggested that glucuronomannan contained alternating 2-linked Man and 4-linked GlcA, while fucoglucuronan contained 4-linked glucuronan with branched Fuc at C-3. Finally, the neuroprotective activities of ST, ST-1, ST-2 and MIX (a mixture of ST-1 and ST-2) were determined. ST showed the most neuroprotective activity, which indicated that ST might be a good candidate for curing neurodegenerative diseases. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[41506165] ; Science and Technology program of applied basic research projects of Qingdao Municipal[15-9-1-74-jch] ; Shandong Provincial Natural Science Foundation, China[ZR2014DQ024]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000425071500037
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158019]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Quanbin; Zhong, Weihong
作者单位	1.Zhejiang Univ Technol, Coll Biotechnol & Bioengineering, Hangzhou 310014, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Jin, Weihua,Liu, Bing,Li, Shuai,et al. The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:307-313.
APA	Jin, Weihua.,Liu, Bing.,Li, Shuai.,Chen, Jing.,Tang, Hong.,...&Zhong, Weihong.(2018).The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,307-313.
MLA	Jin, Weihua,et al."The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):307-313.