Structural and SAXS analysis of Tle5-Tli5 complex reveals a novel inhibition mechanism of H2-T6SS in Pseudomonas aeruginosa

doi:10.1002/pro.3246

	Structural and SAXS analysis of Tle5-Tli5 complex reveals a novel inhibition mechanism of H2-T6SS in Pseudomonas aeruginosa
	She Z(佘准); Dong YH(董宇辉); Gao, ZQ; Wang, WJ; Geng, Z; Xu, JH; She, Z; Dong, YH; Yang, XY; Li, ZQ
刊名	PROTEIN SCIENCE
	2017
卷号	26 期号:10 页码:2083-2091
关键词	X-ray crystallography SAXS immunity protein type VI secretion system
ISSN号	0961-8368
DOI	10.1002/pro.3246
文献子类	Article
英文摘要	Widely spread in Gram-negative bacteria, the type VI secretion system (T6SS) secretes many effector-immunity protein pairs to help the bacteria compete against other prokaryotic rivals, and infect their eukaryotic hosts. Tle5 and Tle5B are two phospholipase effector protein secreted by T6SS of Pseudomonas aeruginosa. They can facilitate the bacterial internalization process into human epithelial cells by interacting with Akt protein of the PI3K-Akt signal pathway. Tli5 and PA5086-5088 are cognate immunity proteins of Tle5 and Tle5B, respectively. They can interact with their cognate effector proteins to suppress their virulence. Here, we report the crystal structure of Tli5 at 2.8 angstrom resolution and successfully fit it into the Small angle X-ray scattering (SAXS) model of the complete Tle5-Tli5 complex. We identified two important motifs in Tli5 through sequence and structural analysis. One is a conserved loop--hairpin motif that exists in the Tle5 immunity homologs, the other is a long and sharp - motif that directly interacts with Tle5 according to SAXS data. We also distinguished the structural features of Tle5 and Tle5B family immunity proteins. Together, our work provided insights into a novel inhibition mechanism that may enhance our understanding of phospholipase D family proteins.
电子版国际标准刊号	1469-896X
WOS关键词	SELF-PROTECTION MECHANISM ; SECRETION SYSTEM ; PROTEIN ; MODEL ; PHOSPHOLIPASES ; SCATTERING ; EFFECTORS ; MEMBRANE ; INSIGHTS ; ENCODES
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000411179200017
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285276]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	She Z,Dong YH,Gao, ZQ,et al. Structural and SAXS analysis of Tle5-Tli5 complex reveals a novel inhibition mechanism of H2-T6SS in Pseudomonas aeruginosa[J]. PROTEIN SCIENCE,2017,26(10):2083-2091.
APA	佘准.,董宇辉.,Gao, ZQ.,Wang, WJ.,Geng, Z.,...&徐建华.(2017).Structural and SAXS analysis of Tle5-Tli5 complex reveals a novel inhibition mechanism of H2-T6SS in Pseudomonas aeruginosa.PROTEIN SCIENCE,26(10),2083-2091.
MLA	佘准,et al."Structural and SAXS analysis of Tle5-Tli5 complex reveals a novel inhibition mechanism of H2-T6SS in Pseudomonas aeruginosa".PROTEIN SCIENCE 26.10(2017):2083-2091.