Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization

doi:10.1002/asia.201700910

	Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization
	Zhao YL(赵宇亮); Li, J., X. Xing, B. Sun, Y. Zhao and Z. Wu; Li JX(李金霞); Xing XQ(邢雪青); Sun BY(孙宝云); Wu ZH(吴忠华)
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2017
卷号	12 期号:20 页码:2646-2651
关键词	cancer therapy iron uptake nanomedicine small-angle X-ray scattering transferrin
ISSN号	1861-4728
DOI	10.1002/asia.201700910
文献子类	Article
英文摘要	Herein, A549 tumor cell proliferation was confirmed to be positively dependent on the concentration of Fe3+ or transferrin (Tf). Gd@C-82(OH)(22) or C-60(OH)(22) effectively inhibited the iron uptake and the subsequent proliferation of A549 cells. The conformational changes of Tf mixed with FeCl3, GdCl3, C-60(OH)(22) or Gd@C-82(OH)(22) were obtained by SAXS. The results demonstrate that Tf homodimers can be decomposed into monomers in the presence of FeCl3, GdCl3 or C-60(OH)(22), but associated into tetramers in the presence of Gd@C-82(OH)(22). The larger change of SAXS shapes between Tf+C-60(OH)(22) and Tf+FeCl3 implies that C-60(OH)(22) is bound to Tf, blocking the iron-binding site. The larger deviation of the SAXS shape from a possible crystal structure of Tf tetramer implies that Gd@C-82(OH)(22) is bound to the Tf tetramer, thus disturbing iron transport. This study well explains the inhibition mechanism of Gd@C-82(OH)(22) and C-60(OH)(22) on the iron uptake and the proliferation of A549 tumor cells and highlights the specific interactions of a nanomedicine with the target biomolecules in cancer therapy.
电子版国际标准刊号	1861-471X
WOS关键词	SMALL-ANGLE SCATTERING ; X-RAY-SCATTERING ; BIOLOGICAL MACROMOLECULES ; FULLERENE DERIVATIVES ; NANOPARTICLES ; CANCER ; GD-AT-C-82(OH)(22) ; ACTIVATION ; PROLIFERATION ; NANOMEDICINE
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000413354300003
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285083]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Zhao YL,Li, J., X. Xing, B. Sun, Y. Zhao and Z. Wu,Li JX,et al. Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization[J]. CHEMISTRY-AN ASIAN JOURNAL,2017,12(20):2646-2651.
APA	赵宇亮,Li, J., X. Xing, B. Sun, Y. Zhao and Z. Wu,李金霞,邢雪青,孙宝云,&吴忠华.(2017).Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization.CHEMISTRY-AN ASIAN JOURNAL,12(20),2646-2651.
MLA	赵宇亮,et al."Metallofullerenol Inhibits Cellular Iron Uptake by Inducing Transferrin Tetramerization".CHEMISTRY-AN ASIAN JOURNAL 12.20(2017):2646-2651.