Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level

doi:10.1021/acsomega.6b00390

	Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level
	Gao XY(高学云); Zhai J(翟皎); Zhao LN(赵丽娜); Zheng LN(郑令娜); Gao FP(高福平); Gao J(高靓); Liu R(刘茹); Wang YL(王亚玲); Zhai, J., L. Zhao, L. Zheng, F. Gao, L. Gao, R. Liu, Y. Wang and X. Gao
刊名	ACS OMEGA
	2017
卷号	2 期号:1 页码:276-282
ISSN号	2470-1343
DOI	10.1021/acsomega.6b00390
文献子类	Article
英文摘要	Alterations in protein (e.g., biomarkers) expression levels have a significant correlation with tumor development and prognosis; therefore, it is desired to develop precise methods to differentiate the expression level of proteins in tumor cell lines, especially at the single-cell level. Here, we report a precise and versatile approach of quantifying the protein expression levels of three tumor cell lines in situ using a peptide-Au cluster probe. The probe (Au(5)Peptide(3)) consists of a peptide with a specific cell membrane epidermal growth factor receptor (EGFR) targeting ability and an Au cluster for both cell membrane EGFR imaging using confocal microscopy and cell membrane EGFR counting by laser ablation inductively coupled plasma mass spectrometry. Utilizing the peptide-Au cluster probe, we successfully quantify the EGFR expression levels of SMMC-7721, KB, and HeLa cells at a single-cell level and differentiate the EGFR expression levels among these cell lines. The peptide-Au cluster probe, with the ability to differentiate the protein expression level of different cell lines, shows exceptional promise for providing reliable predictive and prognostic information of tumors at a single-cell level.
WOS关键词	MASS CYTOMETRY ; HEPATOCELLULAR-CARCINOMA ; NASOPHARYNGEAL CARCINOMA ; PROGNOSTIC-SIGNIFICANCE ; GOLD NANOCRYSTALS ; CURATIVE SURGERY ; CANCER PATIENTS ; EXPRESSION ; EGFR ; IDENTIFICATION
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000395862400034
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285078]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Gao XY,Zhai J,Zhao LN,et al. Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level[J]. ACS OMEGA,2017,2(1):276-282.
APA	高学云.,翟皎.,赵丽娜.,郑令娜.,高福平.,...&Zhai, J., L. Zhao, L. Zheng, F. Gao, L. Gao, R. Liu, Y. Wang and X. Gao.(2017).Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level.ACS OMEGA,2(1),276-282.
MLA	高学云,et al."Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level".ACS OMEGA 2.1(2017):276-282.