Global metabolic responses of the lenok (Brachymystax lenok) to thermal stress

doi:10.1016/j.cbd.2019.01.006

	Global metabolic responses of the lenok (Brachymystax lenok) to thermal stress
	Hao, Fuhua4 ; Lin, Hong 4; Mou, Zhenbo 1; Yin, Jiasheng 5; Liu, Hongbai 5; Bureau, Dominique P.2; Ye, Shaowen 3; Liu, Jiashou 3; Liu, Yang 5
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2019-03-01
卷号	29 页码:308-319
关键词	Thermal stress Metabonomics NMR Multivariate analysis Brachymystax lenok
ISSN号	1744-117X
DOI	10.1016/j.cbd.2019.01.006
英文摘要	High temperature is a powerful stressor for fish living in natural and artificial environments, especially for cold water species. Understanding the impact of thermal stress on physiological processes of fish is crucial for better cultivation and fisheries management. However, the metabolic mechanism of cold water fish to thermal stress is still not completely clear. In this study, a NMR-based metabonomic strategy in combination with high throughput RNA-Seq was employed to investigate global metabolic changes of plasma and liver in a typical cold water fish species lenok (Brachymystax lenok) subjected to a sub-lethal high temperature. Our results showed that thermal stress caused multiple dynamic metabolic alterations of the lenok with prolonged stress, including repression of energy metabolism, shifts in lipid metabolism, alterations in amino acid metabolism, changes in choline and nucleotide metabolisms. Specifically, thermal stress induced an activation of glutamate metabolism, indicating that glutamate could be an important biomarker associated with thermal stress. Evidence from Hsp 70 gene expression, blood biochemistry and histology confirmed that high temperature exposure had negative effects on health of the lenok. These findings imply that thermal stress has a severe adverse effect on fish health and demonstrate that the integrated analyses combining NMR-based metabonomics and transcriptome strategy is a powerful approach to enhance our understanding of metabolic mechanisms of fish to thermal stress.
资助项目	National Natural Science Foundation of China[31502188] ; National Natural Science Foundation of China[21205130] ; China Agriculture Research System ; Natural Science Foundation of Heilongjiang Province of China[QC2015040] ; Central Level Non profit Scientific Research Institutes Special Funds in China[HSY201602] ; China Scholarship Council[201703260016]
WOS关键词	TROUT ONCORHYNCHUS-MYKISS ; MESSENGER-RNA EXPRESSION ; HIGH WATER TEMPERATURE ; RAINBOW-TROUT ; HEAT-STRESS ; GROWTH-PERFORMANCE ; TRANSCRIPTOMIC RESPONSES ; ACCLIMATION TEMPERATURE ; PROTEOMIC ANALYSIS ; IMMUNE-RESPONSES
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000460492500030
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; China Scholarship Council ; China Scholarship Council ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; Central Level Non profit Scientific Research Institutes Special Funds in China ; China Scholarship Council ; China Scholarship Council
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14167]
专题	中国科学院武汉物理与数学研究所
通讯作者	Hao, Fuhua
作者单位	1.Tibet Acad Agr & Anim Husb Sci, Inst Fisheries Sci, Lhasa 850002, Peoples R China 2.Univ Guelph, Dept Anim Biosci, Fish Nutr Res Lab, Guelph, ON N1G 2W1, Canada 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance,Key Lab Magnet Resonan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 5.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Hao, Fuhua,Lin, Hong,Mou, Zhenbo,et al. Global metabolic responses of the lenok (Brachymystax lenok) to thermal stress[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2019,29:308-319.
APA	Hao, Fuhua.,Lin, Hong.,Mou, Zhenbo.,Yin, Jiasheng.,Liu, Hongbai.,...&Liu, Yang.(2019).Global metabolic responses of the lenok (Brachymystax lenok) to thermal stress.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,29,308-319.
MLA	Hao, Fuhua,et al."Global metabolic responses of the lenok (Brachymystax lenok) to thermal stress".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 29(2019):308-319.