Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila

doi:10.1111/jwas.12351

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 其他 > 期刊论文

	Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila
	Dong, Jing 1,2; Ruan, Jing 3; Xu, Ning 1,2; Yang, Yibin 1,2; Ai, Xiaohui 1,2
刊名	JOURNAL OF THE WORLD AQUACULTURE SOCIETY
	2017-06-01
卷号	48 期号:3 页码:531-536
ISSN号	0893-8849
DOI	10.1111/jwas.12351
英文摘要	Aeromonas hydrophila is a major pathogen in aquaculture in China; it is also an emerging pathogen in humans and animals. In recent years, several severe diseases, such as gastroenteritis, meningitis, and skin and soft tissue infections, have been found to be caused by A. hydrophila. Aerolysin and hemolysin are the most important virulence factors secreted by A. hydrophila. Therefore, new drugs targeted at these two toxins can be found in the therapy for A. hydrophila infections, reducing the usage of antibiotics. In this paper, we expressed and purified the aerolysin and hemolysin using a prokaryotic expression system. We found that aerolysin was more toxic than hemolysin, both in vitro and in vivo. The lethal dose (LD50) of aerolysin and hemolysin was 0.4 and 2.3 mu g per fish, respectively.
资助项目	National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS[2015JBFM37] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS[2015JBFM29] ; Special Fund for Agro-Scientific Research in the Public Interest of China[201203085]
WOS关键词	AEROLYSIN ; WATER ; PROAEROLYSIN ; GENES ; MODEL
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000402640300016
资助机构	National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/31806]
专题	水生生物研究所_其他_期刊论文
通讯作者	Dong, Jing; Ai, Xiaohui
作者单位	1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China 2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Dong, Jing,Ruan, Jing,Xu, Ning,et al. Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(3):531-536.
APA	Dong, Jing,Ruan, Jing,Xu, Ning,Yang, Yibin,&Ai, Xiaohui.(2017).Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(3),531-536.
MLA	Dong, Jing,et al."Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.3(2017):531-536.