An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish | |
Xiong, Shuting1; Ma, Wenge1; Jing, Jing1; Zhang, Jin1; Dan, Cheng1; Gui, Jian-Fang1,2; Mei, Jie1 | |
刊名 | ENDOCRINOLOGY
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2018-05-01 | |
卷号 | 159期号:5页码:1982-1991 |
ISSN号 | 0013-7227 |
DOI | 10.1210/en.2018-00015 |
英文摘要 | Besides its well-documented roles in cell proliferation, apoptosis, and carcinogenesis, the function of the p53-microRNA axis has been recently revealed in the reproductive system. Recent studies indicated that miR-200 family members are dysregulated in nonobstructive azoospermia patients, whereas their functions remain poorly documented. The aim of this study was to investigate the function of the miR-200 family on zebrafish testis development and sperm activity. There was no substantial difference in testis morphology and histology between wild-type (WT) and knockout zebrafish with deletion of miR-200 cluster on chromosome 6 (chr6-miR-200-KO) or on chromosome 23 (chr23-miR-200-KO). Interestingly, compared with WT zebrafish, the chr6-miR-200-KO zebrafish had no difference on sperm motility, whereas chr23-miR-200-KO zebrafish showed significantly improved sperm motility. Consistently, ectopic expression of miR-429a, miR-200a, and miR-200b, which are located in the miR-200 cluster on chromosome 23, significantly reduced motility traits of sperm. Several sperm motility-related genes, such as amh, wt1a, and srd5a2b have been confirmed as direct targets of miR-200s on chr23. 17a-ethynylestradiol (EE2) exposure resulted in upregulated expression of p53 and miR-429a in testis and impairment of sperm motility. Strikingly, in p53 mutant zebrafish testis, the expression levels of miR-200s on chr23 were significantly reduced and accompanied by a stimulation of sperm motility. Moreover, the upregulation of miR-429a associated with EE2 treatment was abolished in testis with p53 mutation. And the impairment of sperm activity by EE2 treatment was also eliminated when p53 was mutated. Together, our results reveal that miR-200 cluster on chromosome 23 controls sperm motility in a p53-dependent manner. |
资助项目 | Fundamental Research Funds for the Central Universities[529020900202949] ; National Natural Science Foundation of China[31672635] ; Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDY-SSW-SMC025] |
WOS关键词 | ANTI-MULLERIAN HORMONE ; SPERMATOGONIAL STEM-CELLS ; MALE-INFERTILITY ; SEMINAL PLASMA ; SEX DETERMINATION ; QUALITY-CONTROL ; ANALYSIS CASA ; SELF-RENEWAL ; MICRORNA ; SPERMATOGENESIS |
WOS研究方向 | Endocrinology & Metabolism |
语种 | 英语 |
出版者 | OXFORD UNIV PRESS INC |
WOS记录号 | WOS:000432685100007 |
资助机构 | Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; 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内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/30483] ![]() |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Gui, Jian-Fang; Mei, Jie |
作者单位 | 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Anim Breeding, Minist Agr, Wuhan 430070, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Xiong, Shuting,Ma, Wenge,Jing, Jing,et al. An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish[J]. ENDOCRINOLOGY,2018,159(5):1982-1991. |
APA | Xiong, Shuting.,Ma, Wenge.,Jing, Jing.,Zhang, Jin.,Dan, Cheng.,...&Mei, Jie.(2018).An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish.ENDOCRINOLOGY,159(5),1982-1991. |
MLA | Xiong, Shuting,et al."An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish".ENDOCRINOLOGY 159.5(2018):1982-1991. |
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