Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus) | |
Pang, Meixia1,2; Luo, Weiwei1; Fu, Beide1; Yu, Xiaomu1; Zhou, Ying1,2; Tong, Jingou1 | |
刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
2018-03-01 | |
卷号 | 19期号:3页码:14 |
关键词 | crucian carp (Carassius auratus) feed conversion efficiency brain differentially expressed genes RNA-Seq |
ISSN号 | 1422-0067 |
DOI | 10.3390/ijms19030858 |
英文摘要 | Feed efficiency is an economically crucial trait for cultured animals, however, progress has been scarcely made in the genetic analyses of feed conversion efficiency (FCE) in fish because of the difficulties in measurement of trait phenotypes. In the present investigation, we present the first application of RNA sequencing (RNA-Seq) combined with differentially expressed genes (DEGs) analysis for identification of functional determinants related to FCE at the gene level in an aquaculture fish, crucian carp (Carassius auratus). Brain tissues of six crucian carp with extreme FCE performances were subjected to transcriptome analysis. A total of 544,612 unigenes with a mean size of 644.38 bp were obtained from Low- and High-FCE groups, and 246 DEGs that may be involved in FCE traits were identified in these two groups. qPCR confirmed that genes previously identified as up- or down-regulated by RNA-Seq were effectively up- or down-regulated under the studied conditions. Thirteen key genes, whose functions are associated with metabolism (Dgkk, Mgst3 and Guk1b), signal transduction (Vdnccsalb, Tgf alpha, Nr4a1 and Tacr2) and growth (Endog, Crebrtc2, Myh7, Myh1, Myh14 and Igfbp7) were identified according to GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) annotations. Our novel findings provide useful pathway information and candidate genes for future studies of genetic mechanisms underlying FCE in crucian carp. |
资助项目 | Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences[XDA08010405] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ05] |
WOS关键词 | QUANTITATIVE TRAIT LOCI ; CANDIDATE GENES ; CLIMATE-CHANGE ; BEEF-CATTLE ; GENOME ; IDENTIFICATION ; GROWTH ; FOOD ; PERSPECTIVES ; ASSOCIATION |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000428309800215 |
资助机构 | Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/30434] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Tong, Jingou |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Pang, Meixia,Luo, Weiwei,Fu, Beide,et al. Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(3):14. |
APA | Pang, Meixia,Luo, Weiwei,Fu, Beide,Yu, Xiaomu,Zhou, Ying,&Tong, Jingou.(2018).Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(3),14. |
MLA | Pang, Meixia,et al."Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.3(2018):14. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论