Molecular characterization of caspase members and expression response to Nervous Necrosis Virus outbreak in Pacific cod | |
Mao, Ming-Guang1,2; Jiang, Jie-Lan2; Jiang, Zhi-Qiang2; Liu, Rui-Ting2; Zhang, Qi-Ya1,2; Gui, Jian-Fang1,2 | |
刊名 | FISH & SHELLFISH IMMUNOLOGY |
2018-03-01 | |
卷号 | 74期号:1页码:559-566 |
关键词 | Caspase Pacific cod NNV Apoptosis Development |
ISSN号 | 1050-4648 |
DOI | 10.1016/j.fsi.2018.01.004 |
英文摘要 | Multiple functions of caspases include normal cell turnover, proper development and function of the immune system, hormone-dependent atrophy, embryonic development and chemical-induced cell injury. During artificial propagation of Pacific cod, Gadus macrocephalus, high mortality occurred during early development stages. Here, we performed various analyses on the cDNA and protein sequences of six different G. macrocephalus caspases namely GmCasp3, 6, 7, 8, 9 and 10, and tried to investigate the contributions of caspase family to the development and Nervous Necrosis Virus (NNV) resistance. Sequence analysis of GmCaspase proteins showed that each caspase shared conserved domains like "HG", "QACXG (X for R, G or Q)" and "GSWF" except GmCasp10. Sequence alignment and phylogenetic tree showed that GmCasp8 and GmCasp10 were quite different from those of other fishes. 3-D models indicated that structure of GmCasp3 is very conservative, but GmCasp6, 7, 8, 9 and 10 are less conservative. Tissue distribution analysis showed that six Gmcaspases mRNA transcripts were detected in tissues of intestine, gill, thymus, head-kidney and spleen with different abundance, but Gmcasp7 were not detected in the brain. GmCasp3 transcript was kept at very low level in the early development stages, while the expression levels of GmCasp6, 7, 8, 10 were different at various development stages. GmCasp8 level seemed to be much higher than other caspases in the heads of 65dph and 75dph juveniles. To understand the role of caspases during NNV outbreak, modulation in expression of each Gmcaspases were investigated. The results showed that GmCasp3 transcript level increased significantly when NNV broke out, while GmCasp7, 8, 9 and 10 in cod heads decreased obviously at 69dph and 77dph. The results suggest that caspases in Pacific cod should be diverse in their structure and function, and their unique features and response to NNV outbreak add more evidences for the specificity of immune system in Pacific cod. |
资助项目 | National Natural Science Foundation of China[31302202] ; China Postdoctoral Science Foundation[2016M602391] |
WOS关键词 | CROAKER PSEUDOSCIAENA-CROCEA ; CARP CYPRINUS-CARPIO ; FUNCTIONAL-CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; GADUS-MACROCEPHALUS ; ZEBRAFISH CASPASE-3 ; IMMUNE-SYSTEM ; CLONING ; APOPTOSIS ; GENE |
WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
语种 | 英语 |
出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000427344200060 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 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内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/29663] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Gui, Jian-Fang |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 262306, Peoples R China |
推荐引用方式 GB/T 7714 | Mao, Ming-Guang,Jiang, Jie-Lan,Jiang, Zhi-Qiang,et al. Molecular characterization of caspase members and expression response to Nervous Necrosis Virus outbreak in Pacific cod[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74(1):559-566. |
APA | Mao, Ming-Guang,Jiang, Jie-Lan,Jiang, Zhi-Qiang,Liu, Rui-Ting,Zhang, Qi-Ya,&Gui, Jian-Fang.(2018).Molecular characterization of caspase members and expression response to Nervous Necrosis Virus outbreak in Pacific cod.FISH & SHELLFISH IMMUNOLOGY,74(1),559-566. |
MLA | Mao, Ming-Guang,et al."Molecular characterization of caspase members and expression response to Nervous Necrosis Virus outbreak in Pacific cod".FISH & SHELLFISH IMMUNOLOGY 74.1(2018):559-566. |
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