Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander

doi:10.1186/s12864-018-4596-y

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander
	Ke, Fei; Gui, Jian-Fang; Chen, Zhong-Yuan; Li, Tao; Lei, Cun-Ke; Wang, Zi-Hao; Zhang, Qi-Ya
刊名	BMC GENOMICS
	2018-03-20
卷号	19 期号:1 页码:18
关键词	Interspecies infection Ranavirus Amphibian Transcriptome response Chinese giant salamander Virus-host interactions Immune pathway
ISSN号	1471-2164
DOI	10.1186/s12864-018-4596-y
英文摘要	Background: Ranaviruses (family Iridoviridae, nucleocytoplasmic large DNA viruses) have been reported as promiscuous pathogens of cold-blooded vertebrates. Rana grylio virus (RGV, a ranavirus), from diseased frog Rana grylio with a genome of 105.79 kb and Andrias davidianus ranavirus (ADRV), from diseased Chinese giant salamander (CGS) with a genome of 106.73 kb, contains 99% homologous genes. Results: To uncover the differences in virus replication and host responses under interspecies infection, we analyzed transcriptomes of CGS challenged with RGV and ADRV in different time points (1d, 7d) for the first time. A total of 128,533 unigenes were obtained from 820,858,128 clean reads. Transcriptome analysis revealed stronger gene expression of RGV than ADRV at 1 d post infection (dpi), which was supported by infection in vitro. RGV replicated faster and had higher titers than ADRV in cultured CGS cell line. RT-qPCR revealed the RGV genes including the immediate early gene (RGV-89R) had higher expression level than that of ADRV at 1 dpi. It further verified the acute infection of RGV in interspecies infection. The number of differentially expressed genes and enriched pathways from RGV were lower than that from ADRV, which reflected the variant host responses at transcriptional level. No obvious changes of key components in pathway "Antigen processing and presentation" were detected for RGV at 1 dpi. Contrarily, ADRV infection down-regulated the expression levels of MHC I and CD8. The divergent host immune responses revealed the differences between interspecies and natural infection, which may resulted in different fates of the two viruses. Altogether, these results revealed the differences in transcriptome responses among ranavirus interspecies infection of amphibian and new insights in DNA virus-host interactions in interspecies infection. Conclusion: The DNA virus (RGV) not only expressed self-genes and replicated quickly after entry into host under interspecies infection, but also avoided the over-activation of host responses. The strategy could gain time for the survival of interspecies pathogen, and may provide opportunity for its adaptive evolution and interspecies transmission.
资助项目	National Natural Science Foundation of China[31430091] ; National Natural Science Foundation of China[31772890] ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project[XDA08030202] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01]
WOS关键词	RANA-GRYLIO-VIRUS ; GENE-EXPRESSION DATA ; INFLUENZA-A VIRUSES ; FISH CELL-LINE ; AQUACULTURE ANIMALS ; FAMILY-IRIDOVIRIDAE ; EVASION STRATEGIES ; ENVELOPE PROTEIN ; COMPLEMENT ; EVOLUTION
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000428277300011
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/29632]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Zhang, Qi-Ya
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Ke, Fei,Gui, Jian-Fang,Chen, Zhong-Yuan,et al. Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander[J]. BMC GENOMICS,2018,19(1):18.
APA	Ke, Fei.,Gui, Jian-Fang.,Chen, Zhong-Yuan.,Li, Tao.,Lei, Cun-Ke.,...&Zhang, Qi-Ya.(2018).Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander.BMC GENOMICS,19(1),18.
MLA	Ke, Fei,et al."Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander".BMC GENOMICS 19.1(2018):18.