Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish

doi:10.1016/j.ygcen.2018.08.012

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 淡水生态学研究中心 > 期刊论文

	Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish
	Gong, Yulong 1,2; Zhai, Gang 1; Su, Jingzhi 1,2; Yang, Binyuan 1,2; Jin, Junyan 1; Liu, Haokun 1; Yin, Zhan 1; Xie, Shouqi 1; Han, Dong 1,3
刊名	GENERAL AND COMPARATIVE ENDOCRINOLOGY
	2018-12-01
卷号	269 页码:33-45
关键词	Insulin receptors Blood glucose Hyperglycemia Glycolysis Gluconeogenesis
ISSN号	0016-6480
DOI	10.1016/j.ygcen.2018.08.012
通讯作者	Yin, Zhan(zyin@ihb.ac.cn) ; Han, Dong(hand2lcn@ihb.ac.cn)
英文摘要	An inability of insulin to signal glycolysis and gluconeogenesis would largely result in type 2 diabetes. In this study, the physiological roles of zebrafish insulin receptor a and b in maintaining blood glucose homeostasis were characterized. We observed that, though blood glucose in insra - / - fish and insrb - / - fish were comparable with the control siblings at 0 h postprandium (hpp), the most evident hyperglycemia have been observed in insra - / - fish from 1 hpp to 3 hpp. A mild increase of blood glucose in insrb - / - fish has been seen only at 1.5 hpp. The down-regulated expressions of glycolytic enzymes were observed in insra - / - fish and insrb - / - fish liver and muscle, together with the significantly decreased activities or concentrations of glycolytic enzymes. These results suggest that both Insra and Insrb were critical in glycolysis. Intriguingly, the up-regulated expressions of gluconeogenic enzymes, pck1 and g6pca.1, along with the elevated enzyme activities, were observed in insra - / - fish liver at 1 hpp and 1.5 hpp. Compared with the control fish, the elevated plasma insulin and lowered phosphorylated AKT were observed in insra - / - fish and insrb - / - fish, suggesting that there is an insulin resistance in insra - / - fish and insrb - / - fish. The increased levels of both transcriptions of foxo1a and Foxo1a protein abundance in the insra - / - fish liver have been found. When insra - / - fish treated with the Foxo1 inhibitor, the postprandial blood glucose levels could be normalized, accompanied with the normalized expression levels and enzyme activities of both pck1 and g6pca.1. Therefore, Insra and Insrb demonstrate a similar role in promoting glycolysis, but Insra is involved in inhibiting gluconeogenesis via down-regulating the expression of foxo1a. Our results indicate that Insra and Insrb exhibit diversified functions in maintaining glucose homeostasis in zebrafish.
资助项目	National Basic Research Program of China[2014CB138602] ; Youth Innovation Promotion Association of CAS[2013223] ; National Natural Science Foundation of China[31672670] ; Chinese Academy of Sciences[XDA08010405] ; China Agriculture Research System[CARS -45-09] ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ05]
WOS关键词	TRANSCRIPTION FACTOR FOXO1 ; RAINBOW-TROUT ; BETA-CELL ; GENE-EXPRESSION ; HORMONAL-REGULATION ; FASTING GLYCEMIA ; RESPONSE UNIT ; MICE LACKING ; FACTOR FKHR ; KINASE-B
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000452247100003
资助机构	National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; Youth Innovation Promotion Association of CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology ; Fund Project in State Key Laboratory of Freshwater Ecology and Biotechnology
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28888]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者	Yin, Zhan; Han, Dong
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Gong, Yulong,Zhai, Gang,Su, Jingzhi,et al. Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2018,269:33-45.
APA	Gong, Yulong.,Zhai, Gang.,Su, Jingzhi.,Yang, Binyuan.,Jin, Junyan.,...&Han, Dong.(2018).Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish.GENERAL AND COMPARATIVE ENDOCRINOLOGY,269,33-45.
MLA	Gong, Yulong,et al."Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish".GENERAL AND COMPARATIVE ENDOCRINOLOGY 269(2018):33-45.