Secretogranin-II plays a critical role in zebrafish neurovascular modeling

doi:10.1093/jmcb/mjy027

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Secretogranin-II plays a critical role in zebrafish neurovascular modeling
	Tao, Binbin 1,2; Hu, Hongling 1,2; Mitchell, Kimberly 3; Chen, Ji 1; Jia, Haibo 4; Zhu, Zuoyan 1; Trudeau, Vance L.3; Hu, Wei 1
刊名	JOURNAL OF MOLECULAR CELL BIOLOGY
	2018-10-01
卷号	10 期号:5 页码:388-401
关键词	Secretogranin-II secretoneurin TALENs neurovascular zebrafish
ISSN号	1674-2788
DOI	10.1093/jmcb/mjy027
通讯作者	Trudeau, Vance L.(trudeauv@uottawa.ca) ; Hu, Wei(huwei@ihb.ac.cn)
英文摘要	Secretoneurin (SN) is a neuropeptide derived from specific proteolytic processing of the precursor secretogranin II (SgII). In zebrafish and other teleosts, there are two paralogs named sgIIa and sgIIb. Our results showed that neurons expressing sgIIb were aligned with central arteries in the hindbrain, demonstrating a close neurovascular association. Both sgIIb(-/-) and sgIIa(-/-)/sgIIb(-/-) mutant embryos were defective in hindbrain central artery development due to impairment of migration and proliferation of central artery cells. Further study revealed that sgIIb is non-cell autonomous and required for central artery development. Hindbrain arterial and venous network identities were not affected in sgIIb(-/-) mutant embryos, and the mRNA levels of Notch and VEGF pathway-related genes were not altered. However, the activation of MAPK and PI3K/AKT pathways was inhibited in sgIIb(-/-) mutant embryos. Reactivation of MAPK or PI3K/AKT in endothelial cells could partially rescue the central artery developmental defects in the sgIIb mutants. This study provides the first in vivo evidence that sgIIb plays a critical role in neurovascular modeling of the hindbrain. Targeting the SgII system may, therefore, represent a new avenue for the treatment of vascular defects in the central nervous system.
资助项目	National Natural Science Foundation of China[31325026] ; National Natural Science Foundation of China[31721005] ; Natural Sciences and Engineering Research Council of Canada ; University of Ottawa International Research Acceleration Program
WOS关键词	BLOOD-VESSELS ; SECRETONEURIN ; ANGIOGENESIS ; NEUROPEPTIDE ; MECHANISMS ; CANCER ; NOTCH ; RAT ; GRIDLOCK ; RECEPTOR
WOS研究方向	Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000455315700003
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; Natural Sciences and Engineering Research Council of Canada ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28699]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Trudeau, Vance L.; Hu, Wei
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada 4.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Tao, Binbin,Hu, Hongling,Mitchell, Kimberly,et al. Secretogranin-II plays a critical role in zebrafish neurovascular modeling[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2018,10(5):388-401.
APA	Tao, Binbin.,Hu, Hongling.,Mitchell, Kimberly.,Chen, Ji.,Jia, Haibo.,...&Hu, Wei.(2018).Secretogranin-II plays a critical role in zebrafish neurovascular modeling.JOURNAL OF MOLECULAR CELL BIOLOGY,10(5),388-401.
MLA	Tao, Binbin,et al."Secretogranin-II plays a critical role in zebrafish neurovascular modeling".JOURNAL OF MOLECULAR CELL BIOLOGY 10.5(2018):388-401.