Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi

doi:10.1016/j.dci.2019.03.007

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi
	Huo, Hui Jun 1,2,3; Chen, Shan Nan 1,2; Li, Li 1,2; Laghari, Zubair Ahmed 1,2; Li, Nan 1,2; Nie, Pin 1,2,4,5
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2019-08-01
卷号	97 页码:88-97
关键词	IL-22 IL-22BP Hepcidin LEAP-2 Mandarin fish
ISSN号	0145-305X
DOI	10.1016/j.dci.2019.03.007
通讯作者	Nie, Pin(pinnie@ihb.ac.cn)
英文摘要	As an important immune regulatory molecule, interleukin (IL)-22 has been reported in several species of fish, but its soluble receptor, IL 22 binding protein (IL-22BP), discovered as a natural antagonist of IL-22 in mammals, has not been functionally characterized in fish to date. In the present study, IL-22 and IL-22BP genes were cloned in mandarin fish Siniperca chuatsi. They all exhibited a high basal expression level in mucosa-enriched tissues, implying their possible roles in mucosal immunity. The IL-22 was found to show a potent response to LPS stimulation, acting as an inducer of antimicrobial peptide (AMP) genes, such as hepcidin and Liver-expressed antimicrobial peptide-2 (LEAP-2) in intestinal cells. IL-22BP, via co-incubation with IL-22, inhibited completely the induction of downstream genes by IL-22. Through a yeast two-hybrid assay, the interaction between IL-22BP and IL-22 was confirmed, which may account for the inhibitory effect of IL-22BP. Moreover, two hot spot residues for IL-22 binding, as reported in mammalian IL-22BP, were found to be conserved both in sequence location and function in mandarin fish IL-22BP, indicating that the interaction mode between IL-22 and IL-22BP may be also conserved in fish and mammals. In conclusion, the mandarin fish IL-22 and IL-22BP are conserved in their interaction and function with their mammalian orthologues, and these findings provide basis for future research on IL-22-IL-22BP axis in fish immunity.
资助项目	National Natural Science Foundation of China[30230075] ; China Agriculture Research System[CARS-46] ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China
WOS关键词	T-CELL ; EXPRESSION ANALYSIS ; CRYSTAL-STRUCTURE ; INDUCIBLE FACTOR ; GENE-EXPRESSION ; IL-10 FAMILY ; IMMUNE CELLS ; HOST-DEFENSE ; IL-TIF ; CYTOKINE
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000468721600010
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28489]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Nie, Pin
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Huo, Hui Jun,Chen, Shan Nan,Li, Li,et al. Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,97:88-97.
APA	Huo, Hui Jun,Chen, Shan Nan,Li, Li,Laghari, Zubair Ahmed,Li, Nan,&Nie, Pin.(2019).Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,97,88-97.
MLA	Huo, Hui Jun,et al."Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 97(2019):88-97.