Receptor complex and signalling pathway of the two type II IFNs, IFN-gamma and IFN-gamma rel in mandarin fish or the so-called Chinese perch Siniperca chuatsi

doi:10.1016/j.dci.2019.03.016

	Receptor complex and signalling pathway of the two type II IFNs, IFN-gamma and IFN-gamma rel in mandarin fish or the so-called Chinese perch Siniperca chuatsi
	Li, Li 1,2,3; Chen, Shan Nan 1,2; Laghari, Zubair Ahmed 1,2; Huang, Bei 1,2,6; Huo, Hui Jun 1,2; Li, Nan 1,2; Nie, P.1,2,4,5
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2019-08-01
卷号	97 页码:98-112
关键词	IFN-gamma IFN-gamma rel CRFB17 CRFB13 CRFB6 Mandarin fish
ISSN号	0145-305X
DOI	10.1016/j.dci.2019.03.016
通讯作者	Nie, P.(pinnie@ihb.ac.cn)
英文摘要	IFN-gamma, as the sole member of mammalian type II IFN, is a multifunctional cytokine which exerts its effects through two distinct IFN-gamma receptors, IFNGR1 and IFNGR2. However, in teleost fish, another IFN-gamma homologous gene, namely IFN-gamma related gene (IFN-gamma rel), has been identified. Although IFN-gamma and IFN-gamma rel genes have been described in some fish species, many important aspects remain poorly understood in relation with their signalling and function. In the present study, IFN-gamma and IFN-gamma rel, as well as their receptors, cytokine receptor family B (CRFB) 17, CRFB13, two of which are homologous to IFNGR1 in mammals, and CRFB6, homolomous to IFNGR2, have been characterized in mandarin fish, Siniperca chuatsi. It was revealed that the two type IFN members exhibit antiviral activity, and IFN-gamma transduces downstream signalling through CRFB13 and CRFB6, while IFN-gamma rel interacts with CRFB17 to activate downstream signalling. Moreover, IFN-gamma and IFN-gamma rel have been shown to exert antiviral biological activity in a STAT1-dependent manner. Intracellular domain analysis of CRFB17 and CRFB13 demonstrated that the Y386 tyrosine residue of CRFB13 is required for the activation of the IFN-gamma-mediated biologic response, and the Y324 and Y370 residues in CRFB17 are required to activate IFN-gamma rel signalling.
资助项目	National Natural Science Foundation of China[30230075] ; China Agriculture Research System[CARS-46] ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China
WOS关键词	DISTINCT INTERFERON-GAMMA ; EXPRESSION ANALYSIS ; FUNCTIONAL-CHARACTERIZATION ; MOLECULAR CHARACTERIZATION ; TYROSINE PHOSPHORYLATION ; NECROSIS VIRUS ; CELL-LINE ; IDENTIFICATION ; STAT1 ; MECHANISMS
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000468721600011
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishi Yiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28097]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心
通讯作者	Nie, P.
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China 6.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Li, Li,Chen, Shan Nan,Laghari, Zubair Ahmed,et al. Receptor complex and signalling pathway of the two type II IFNs, IFN-gamma and IFN-gamma rel in mandarin fish or the so-called Chinese perch Siniperca chuatsi[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,97:98-112.
APA	Li, Li.,Chen, Shan Nan.,Laghari, Zubair Ahmed.,Huang, Bei.,Huo, Hui Jun.,...&Nie, P..(2019).Receptor complex and signalling pathway of the two type II IFNs, IFN-gamma and IFN-gamma rel in mandarin fish or the so-called Chinese perch Siniperca chuatsi.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,97,98-112.
MLA	Li, Li,et al."Receptor complex and signalling pathway of the two type II IFNs, IFN-gamma and IFN-gamma rel in mandarin fish or the so-called Chinese perch Siniperca chuatsi".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 97(2019):98-112.