SVCV infection triggers fish IFN response through RLR signaling pathway

doi:10.1016/j.fsi.2018.12.063

	SVCV infection triggers fish IFN response through RLR signaling pathway
	Gong, Xiu-Ying 1,2; Zhang, Qi-Min 1,2; Gui, Jian-Fang 1,2; Zhang, Yi-Bing 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019-03-01
卷号	86 页码:1058-1063
关键词	RNA virus infection Fish IFN response RLR receptor Signaling pathway
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.12.063
通讯作者	Zhang, Yi-Bing(ybzhang@ihb.ac.cn)
英文摘要	In mammals, virus infection of host cells triggers innate immune response, characterized by induction of interferon (IFN) and downstream IFN-stimulated genes (ISGs). The initiation of IFN antiviral response is dependent on host recognition of virus infection. In fish, similar IFN antiviral response is induced in response to RNA or DNA virus infection; however, the detailed mechanisms underlying recognition of a given virus and activation of downstream signaling remain largely unexplored. Using an infection model with Epithelioma papulosum cyprini (EPC) cells and spring viremia of carp virus (SVCV), a negative sense single-stranded RNA virus, we reported that fish RLR signaling pathway was involved in SVCV-triggered fish IFN response. IFN response was significantly initiated in EPC cells when infected with SVCV, as evidenced by activation of fish IFN promoters, upregulation of IFN and ISGs at mRNA and protein levels. However, function blockade of RIG-I and MDA5, two cytosolic receptors of fish RLR family, significantly attenuated the activation of fish IFN promoters and also the induction of fish IFN and ISGs by SVCV infection. Consistently, SVCV infection-triggered IFN response were blocked in EPC cells when transfected with the dominant negative mutants of pivotal RLR signaling factors, including MAVS, MITA, TBK1, IRF3 and IRF7. These results together shed light on the conservation of RLR-mediated IFN signaling that contributes to fish cells responding to RNA virus infection.
资助项目	National Key R&D Program of China[2018YFD0900302] ; National Natural Science Foundation of China[31572646] ; National Natural Science Foundation of China[31772875]
WOS关键词	ANTIVIRAL RESPONSE ; RIG-I ; SPRING VIREMIA ; ZEBRAFISH IRF1 ; RECEPTORS ; BINDING ; PROTEIN ; EXPRESSION ; IFN-PHI-1 ; REVEALS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000459528400117
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28059]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心
通讯作者	Zhang, Yi-Bing
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Minist Agr,Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
推荐引用方式 GB/T 7714	Gong, Xiu-Ying,Zhang, Qi-Min,Gui, Jian-Fang,et al. SVCV infection triggers fish IFN response through RLR signaling pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1058-1063.
APA	Gong, Xiu-Ying,Zhang, Qi-Min,Gui, Jian-Fang,&Zhang, Yi-Bing.(2019).SVCV infection triggers fish IFN response through RLR signaling pathway.FISH & SHELLFISH IMMUNOLOGY,86,1058-1063.
MLA	Gong, Xiu-Ying,et al."SVCV infection triggers fish IFN response through RLR signaling pathway".FISH & SHELLFISH IMMUNOLOGY 86(2019):1058-1063.