Mechanism of intramolecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine

CORC > 化学研究所 > 中国科学院化学研究所

	Mechanism of intramolecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine
	Zhang, HJ; Wang, P; Wang, XF; Feng, J; Xu, SC; Al, X; Zhang, XK; Zhang, JP
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2004-10-01
卷号	47 期号:5 页码:367-372
关键词	2-aminopurine (2ap) Dna Charge Transfer Time-resolved Fluorescence Spectroscopy
ISSN号	1006-9291
英文摘要	As a structural analogue of adenine, 2-aminopurine (2Ap) is often used as a fluorescent probe to study the intramolecular charge transfer reaction in DNA. We have designed and synthesized a series of model DNA helix with the variation in the distance between the 2Ap probe and the GGG sequence, and have investigated, by means of picosecond time-resolved fluorescence spectroscopy, the effect of the length of the bridge (consisting of a number of inosines, 1) separating the electron donor (... GGG...) and the acceptor (2Ap) on the charge transfer dynamics. The fluorescence dynamics of 2Ap exhibited three exponential decay components, the one with a time constant of a few hundred picoseconds is assigned to the intramolecular charge transfer from GGG to 2Ap. Within 2.4 nm of the donor-acceptor separation, the rate of charge transfer decreased exponentially upon increasing the separation, from which the decay factor beta is determined to be 1.3 nm(-1). Beyond 2.4 nm, however, the rate started to increase, this abnormal behavior of charge transfer is interpreted in terms of the match of electronic energies between the 1-bridge and the donor/acceptor couple.
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000225425000002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/81933]
专题	中国科学院化学研究所
通讯作者	Al, X
作者单位	Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhang, HJ,Wang, P,Wang, XF,et al. Mechanism of intramolecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2004,47(5):367-372.
APA	Zhang, HJ.,Wang, P.,Wang, XF.,Feng, J.,Xu, SC.,...&Zhang, JP.(2004).Mechanism of intramolecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine.SCIENCE IN CHINA SERIES B-CHEMISTRY,47(5),367-372.
MLA	Zhang, HJ,et al."Mechanism of intramolecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine".SCIENCE IN CHINA SERIES B-CHEMISTRY 47.5(2004):367-372.