A novel fluorescent probe for selective labeling of histidine

doi:10.1016/j.aca.2004.03.008

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	A novel fluorescent probe for selective labeling of histidine
	Li, XH; Ma, HM; Nie, LH; Sun, M; Xiong, SX
刊名	ANALYTICA CHIMICA ACTA
	2004-07-12
卷号	515 期号:2 页码:255-260
关键词	Fluorescent Probe Histidine Labeling 1-(2 3-epoxypropoxy)-2-phenylazo-8-acetylaminonaphthalene-3 6-disulfonic Acid Sodium Salt
ISSN号	0003-2670
DOI	10.1016/j.aca.2004.03.008
英文摘要	A novel water-soluble fluorescent probe, 1-(2,3-epoxypropoxy)-2-phenylazo-8-acetylamino-naphthalene-3,6-disulfonic acid sodium salt (EPANS), was designed and synthesized by reacting epichlorohydrin with azophloxine, and its properties for labeling of histidine were studied. In alkaline media, EPANS reacted selectively with histidine rather than with other amino acids, causing a large decrease in fluorescence intensity and thereby allowing a selective detection of histidine without a tedious separation. The linear range was 4-500 muM with a detection limit of 1 muM (S/N = 3). The proposed method has been successfully used to determine histidine in human serum. The obtained results were in good agreement with those given by using histidine-nickel complex adsorptive voltammetry. (C) 2004 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000222370100002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/81695]
专题	中国科学院化学研究所
通讯作者	Ma, HM
作者单位	Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Biol Chem Lab, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Li, XH,Ma, HM,Nie, LH,et al. A novel fluorescent probe for selective labeling of histidine[J]. ANALYTICA CHIMICA ACTA,2004,515(2):255-260.
APA	Li, XH,Ma, HM,Nie, LH,Sun, M,&Xiong, SX.(2004).A novel fluorescent probe for selective labeling of histidine.ANALYTICA CHIMICA ACTA,515(2),255-260.
MLA	Li, XH,et al."A novel fluorescent probe for selective labeling of histidine".ANALYTICA CHIMICA ACTA 515.2(2004):255-260.