A new method to improve sensitivity and resolution in matrix-assisted laser desorption/ionization time of flight mass spectrometry

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	A new method to improve sensitivity and resolution in matrix-assisted laser desorption/ionization time of flight mass spectrometry
	Xiong, SX ; Ding, QX ; Zhao, ZW ; Chen, WZ ; Wang, GH ; Liu, SJ
刊名	PROTEOMICS
	2003-03-01
卷号	3 期号:3 页码:265-272
关键词	Hydrophobic Membrane Matrix-assisted Laser Desorption/ionization Time Of Flight Mass Spectrometry Resolution Sensitivity Silver Staining
ISSN号	1615-9853
英文摘要	High detection sensitivity and resolution are two critical parameters for recording good peptide mass fingerprints (PMF) of low abundance proteins. This paper reports a mass spectrometry (MS) sample preparation technique that could improve sensitivity and resolution. By coating the MS steel target with a thin layer of pentadecafluorooctamido propyltrimethoxysilane, which was both polar and nonpolar solvent repellent, the transferred sample droplets on its surface were significantly smaller. As a result, the analyte of the peptide mixture became more concentrated and homogeneous, which helped to improve the sensitivity. The advantages of a modified MS target were documented by mass spectra improvement of attomole level standard peptides and silver-stained proteins from polyacrylamide gels. The mass signal of angiotensin II at 100 attomole was difficult to record on the conventional support, whereas it was easily detected on the modified one. The PMF of cytochrome C was also better recorded on the modified support, in terms of both signal-to-noise ratio and the number of detected peptides. When silver-stained proteins from two-dimensional electrophoresis gels were analyzed, in most cases more satisfactory peptide mass spectra were obtained from the modified support. Searching protein databases with more mass data from the improved PMFs, several unknown proteins were successfully identified.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000181700000004
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/80835]
专题	中国科学院化学研究所
通讯作者	Liu, SJ
作者单位	1.Inst Basic Med Sci, Dept Neurobiol, Beijing 100850, Peoples R China 2.Ctr Mol Sci, Inst Chem, Beijing Mass Spectrometry Ctr, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xiong, SX,Ding, QX,Zhao, ZW,et al. A new method to improve sensitivity and resolution in matrix-assisted laser desorption/ionization time of flight mass spectrometry[J]. PROTEOMICS,2003,3(3):265-272.
APA	Xiong, SX,Ding, QX,Zhao, ZW,Chen, WZ,Wang, GH,&Liu, SJ.(2003).A new method to improve sensitivity and resolution in matrix-assisted laser desorption/ionization time of flight mass spectrometry.PROTEOMICS,3(3),265-272.
MLA	Xiong, SX,et al."A new method to improve sensitivity and resolution in matrix-assisted laser desorption/ionization time of flight mass spectrometry".PROTEOMICS 3.3(2003):265-272.