The position investigation of the substituted hydroxyethyl groups of hydroxyethyl starch

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	The position investigation of the substituted hydroxyethyl groups of hydroxyethyl starch
	Liu, YM ; Xu, GY ; He, L ; Jiang, CG ; Li, JC ; Song, G ; Mei, SC
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2003-03-01
卷号	31 期号:3 页码:271-276
关键词	Starch Hydroxyethyl Positions Of Substituted Gas Chromatography/mass Spectrometry
ISSN号	0253-3820
英文摘要	Hydroxyethyl starch (HES) is currently finding increasing rise as a replacement for plasma. It is desirable to have a precise knowledge of the positions and the categories of the substituted hydroxyethyl groups in clinical applications, as it affects the pharmacokinetics and pharmacology. The Study of the positions of the substituted hydroxyethyl groups involves the conversion of all free hydroxyls in the original HES into methoxyl groups, followed by hydrolytic cleavage of the glycosidic linkages with acid and the conversion of methylated sugar into alditol acetates. The derivatives have been studied by gas chromatography/chemical ionization mass spectrometry (GC/CIMS) and GC/electron impact mass spectrometry (EIMS)/MS. Investigations concerning the EI fragmentation schemes allowed secure determinations of the positions and the categories of the substituted hydroxyethyl groups, especially determinations of monohydroxyethyl derivatives and their differentiations in the EI fragmentation schemes. CI measurements permitted the recognition of introduced hydroxyethyl groups in the glucose residues by detection of (M+1)(+)-60 signals. Proposed generations of the main fragment ions are presented. According to the relative peak areas of the signals in the total ion chromatogram, the quantity of substitution is 36.5% and the ratio of the substition at C-2 is 82.0%.
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000183030400004
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/79955]
专题	中国科学院化学研究所
通讯作者	Xu, GY
作者单位	1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Engn Plast, Beijing 100080, Peoples R China 2.Gen Hosp Peoples Liberat Army, Beijing 100853, Peoples R China
推荐引用方式 GB/T 7714	Liu, YM,Xu, GY,He, L,et al. The position investigation of the substituted hydroxyethyl groups of hydroxyethyl starch[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(3):271-276.
APA	Liu, YM.,Xu, GY.,He, L.,Jiang, CG.,Li, JC.,...&Mei, SC.(2003).The position investigation of the substituted hydroxyethyl groups of hydroxyethyl starch.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(3),271-276.
MLA	Liu, YM,et al."The position investigation of the substituted hydroxyethyl groups of hydroxyethyl starch".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.3(2003):271-276.