Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes

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	Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes
	Yu, CL; Chen, S; Zhang, MH; Shen, T
刊名	PHOTOCHEMISTRY AND PHOTOBIOLOGY
	2001-05-01
卷号	73 期号:5 页码:482-488
ISSN号	0031-8655
英文摘要	Hypocrellin B (HB), a lipid-soluble natural pigment of perylenequinone derivative, is considered as potential photosensitizer for photodynamic therapy. Liposomes loaded with HE can constitute a simple model system, appropriate for better understanding the photodynamic action of HE in vivo. The steady-state absorption and emission spectra, quantum yield and lifetime of fluorescence of HE incorporated into egg L-a-phosphatidyl-choline (EPC) liposome were examined, The photochemical properties (Type I and/or Type II) of HE have also been studied in aqueous dispersions of small unilamellar liposomes of EPC using electron paramagnetic resonance and spectrophotometric methods, respectively. The quantum yield of O-1(2) generated by HE is ca 0.76 in chloroform solution and it did not change upon the incorporation of HE into liposomes of EPC, The superoxide anion radical was generated by the electron transfer from the anion radical of HE (HB.-) to oxygen. The disproportionation of O-2(.-) can generate H2O2 and ultimately the highly reactive (OH)-O-. via the Fenton reaction. It could be that the disproportionation proceeded too fast, so we could not detect O-2(.-) directly in aqueous dispersions of liposome EPC, Moreover, the self-sensitized photooxygenation of HE embedded in liposomes was studied, and almost fully (87%) inhibiting this reaction of HE by p-benzoquinone las the quencher of O-2(.-)) in aqueous dispersion of liposome EPC indicated that the radical mechanism (Type I) might be mainly involved in this oxygenation. All these findings suggested that the photodynamic action of HE proceeded via both Type-I and -II mechanisms, but Type-I mechanism might play a more important role in the aqueous dispersion.
语种	英语
出版者	AMER SOC PHOTOBIOLOGY
WOS记录号	WOS:000168578400005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/77167]
专题	中国科学院化学研究所
通讯作者	Zhang, MH
作者单位	Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Yu, CL,Chen, S,Zhang, MH,et al. Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY,2001,73(5):482-488.
APA	Yu, CL,Chen, S,Zhang, MH,&Shen, T.(2001).Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes.PHOTOCHEMISTRY AND PHOTOBIOLOGY,73(5),482-488.
MLA	Yu, CL,et al."Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes".PHOTOCHEMISTRY AND PHOTOBIOLOGY 73.5(2001):482-488.