Monolith and coating enzymatic microreactors of L-asparaginase: kinetics study by MCE-LIF for potential application in acute lymphoblastic leukemia (ALL) treatment

doi:10.1039/c1an15067g

CORC > 化学研究所 > 中国科学院化学研究所

	Monolith and coating enzymatic microreactors of L-asparaginase: kinetics study by MCE-LIF for potential application in acute lymphoblastic leukemia (ALL) treatment
	Qiao, Juan 1; Qi, Li 1; Mu, Xiaoyu 1,2; Chen, Yi 1
刊名	ANALYST
	2011
卷号	136 期号:10 页码:2077-2083
ISSN号	0003-2654
DOI	10.1039/c1an15067g
英文摘要	The study of enzyme immobilization using an extracorporeal shunt system is essential to eliminate the side effects of L-asparaginase (L-Asnase; including hepatic toxicity, allergic reaction, pancreatitis, central nervous system toxicity and decreased synthesis of blood clotting factors) when it was applied as an anticancer drug given directly to patients by injection. Thus, the novel monolith and coating enzymatic reactors of L-asparaginase were provided in this assay and a microchip electrophoresis-laser induced fluorescence (MCE-LIF) method was set up for the enzyme kinetics study. The enzymatic reactors would be a promising in vitro therapeutic method in an extracorporeal shunt system for acute lymphoblastic leukemia (ALL) treatment. For the first time, L-asparaginase was covalently bound to the polymer monolith and coating in the capillary and the activity characteristics of these enzymatic microreactors have been probed by Michaelis-Menten kinetic constants. Meanwhile, the D, L-amino acids were chirally separated using microchip electrophoresis with a laser induced detector and D, Laspartic acid (D,L-Asp) were tested for the L-asparaginase enzymatic reactor kinetics study. Furthermore, human serum adding with L-asparagine (L-Asn) as the sample was hydrolyzed by the enzymatic microreactors. The results demonstrated that the developed enzymatic microreactor of L-asparaginase would be a potential therapeutic protocol for ALL treatment.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000289896000010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/73141]
专题	中国科学院化学研究所
通讯作者	Qi, Li
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Juan,Qi, Li,Mu, Xiaoyu,et al. Monolith and coating enzymatic microreactors of L-asparaginase: kinetics study by MCE-LIF for potential application in acute lymphoblastic leukemia (ALL) treatment[J]. ANALYST,2011,136(10):2077-2083.
APA	Qiao, Juan,Qi, Li,Mu, Xiaoyu,&Chen, Yi.(2011).Monolith and coating enzymatic microreactors of L-asparaginase: kinetics study by MCE-LIF for potential application in acute lymphoblastic leukemia (ALL) treatment.ANALYST,136(10),2077-2083.
MLA	Qiao, Juan,et al."Monolith and coating enzymatic microreactors of L-asparaginase: kinetics study by MCE-LIF for potential application in acute lymphoblastic leukemia (ALL) treatment".ANALYST 136.10(2011):2077-2083.