Surface-initiated molecularly imprinted polymeric column: In situ synthesis and application for semi-preparative separation by high performance liquid chromatography

doi:10.1016/j.chroma.2011.09.019

CORC > 化学研究所 > 中国科学院化学研究所

	Surface-initiated molecularly imprinted polymeric column: In situ synthesis and application for semi-preparative separation by high performance liquid chromatography
	Gu, Jiangyong 1; Zhang, Hu 1,2; Yuan, Gu 1; Chen, Lirong 1; Xu, Xiaojie 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2011-11-11
卷号	1218 期号:45 页码:8150-8155
关键词	Molecularly Imprinted Polymer In Situ Synthesis Hplc Semi-preparative Emodin
ISSN号	0021-9673
DOI	10.1016/j.chroma.2011.09.019
英文摘要	In this work, we prepared a monolithic and surface initiated molecularly imprinted polymeric (MIP) column for HPLC and explored its application for template separation from plant extract. The silica beads (40-60 mu m) were coupled with initiator on the surface and then packed in to a stainless steel HPLC column. The pre-polymerization mixture (the template, functional monomer and crosslinker were emodin, acrylamide and divinylbenzene, respectively) was injected into the column and polymerized by thermal initiation. The prepared MIP column exhibited excellent retention capability and large imprinted factor for template (the retention time and imprinted factor for emodin on MIP column were 16.26 min and 7.21, respectively). Moreover, the emodin-molecularly imprinted polymeric column could be applied to separate emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale and 1.2 mg of emodin was obtained in 4h. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000296937400003
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/73093]
专题	中国科学院化学研究所
通讯作者	Chen, Lirong
作者单位	1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Zhengzhou Univ, Dept Chem, Zhengzhou 450052, Peoples R China
推荐引用方式 GB/T 7714	Gu, Jiangyong,Zhang, Hu,Yuan, Gu,et al. Surface-initiated molecularly imprinted polymeric column: In situ synthesis and application for semi-preparative separation by high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(45):8150-8155.
APA	Gu, Jiangyong,Zhang, Hu,Yuan, Gu,Chen, Lirong,&Xu, Xiaojie.(2011).Surface-initiated molecularly imprinted polymeric column: In situ synthesis and application for semi-preparative separation by high performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1218(45),8150-8155.
MLA	Gu, Jiangyong,et al."Surface-initiated molecularly imprinted polymeric column: In situ synthesis and application for semi-preparative separation by high performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1218.45(2011):8150-8155.