In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection

doi:10.1002/jssc.201100551

CORC > 化学研究所 > 中国科学院化学研究所

	In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection
	Bai, Yu; Du, Fuyou; Yang, Youyou; Bai, Yu; Liu, Huwei
刊名	JOURNAL OF SEPARATION SCIENCE
	2011-10-01
卷号	34 期号:20 页码:2893-2900
关键词	Ce Non-covalent Labeling Plant Peptide Hormone Quantum Dots Systemin
ISSN号	1615-9306
DOI	10.1002/jssc.201100551
英文摘要	Quantum dots (QDs), with their superior size-dependent fluorescence properties, have been employed as non-covalent fluorescent labels for the determination of tomato systemin (TomSys) by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. The optimum conditions of in-capillary labeling and CE separation were investigated in detail, and complete separation of QDs-labeled TomSys from free QDs labels was achieved. Satisfactory results were obtained in terms of linearity (R(2) = 0.998), sensitivity (limit of detection, 66 fmol) and repeatability (run-to-run RSDs of migration time and peak area, 0.9 and 4.6%, respectively; day-to-day RSDs of migration time and peak area, 3.1 and 11.9%, respectively). The established CE-LIF method was later applied in the detection of TomSys spiked in the sample of tomato leaves, which showed the applicability of the proposed method in the analysis of the target plant peptide hormone in the complex matrix.
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000296905400015
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/72535]
专题	中国科学院化学研究所
通讯作者	Liu, Huwei
作者单位	Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Key Lab Bioorgan Chem & Mol Engn,Inst Analyt Chem, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Bai, Yu,Du, Fuyou,Yang, Youyou,et al. In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(20):2893-2900.
APA	Bai, Yu,Du, Fuyou,Yang, Youyou,Bai, Yu,&Liu, Huwei.(2011).In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection.JOURNAL OF SEPARATION SCIENCE,34(20),2893-2900.
MLA	Bai, Yu,et al."In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection".JOURNAL OF SEPARATION SCIENCE 34.20(2011):2893-2900.