Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis

doi:10.1002/rcm.5055

CORC > 化学研究所 > 中国科学院化学研究所

	Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis
	Sun, Shutao 1,2; Ma, Haitao 3; Han, Guanghui 2; Wu, Ren'an 2; Zou, Hanfa 2; Liu, Yiwei 1
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2011-07-15
卷号	25 期号:13 页码:1862-1868
ISSN号	0951-4198
DOI	10.1002/rcm.5055
英文摘要	An efficient and simple method for enrichment and identification of phosphopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) using cerium oxide is presented. After pretreatment of tryptic digests of phosphoproteins with CeO(2), nonphosphopeptides are discarded and phosphopeptides are enriched. By applying the separated CeO(2) on a target plate and analysis using MALDI-TOF MS, peaks of phosphopeptides and their correspondingly series of dephosphorylated peptides are observed in the mass spectra. Thus, the phosphopeptides are very easy to identify with the mass difference, which are all 80 Da between adjacent peaks in the same series, and clear background in the spectra owing to elimination of signal suppression from large amounts of nonphosphopeptides. Furthermore, the phosphopeptides can be dephosphorylated completely after a further NH(4)OH elution. Tryptic digest products from several standard proteins are pretreated using CeO(2) to demonstrate the efficiency of this method. Phosphopeptides from a very small quantity of human serum are enriched and analyzed, and proteins also identified by searching against a database using Mascot on MALDI-TOF/TOF fragments, which indicates that this method may be employed in complex samples for further application. Copyright (C) 2011 John Wiley & Sons, Ltd.
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000291593900010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/72067]
专题	中国科学院化学研究所
通讯作者	Sun, Shutao
作者单位	1.Chinese Acad Sci, Inst Microbiol, Lab Struct Microbiol, Core Facil, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.Chinese Acad Sci, State Key Lab Mol React Dynam, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Sun, Shutao,Ma, Haitao,Han, Guanghui,et al. Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2011,25(13):1862-1868.
APA	Sun, Shutao,Ma, Haitao,Han, Guanghui,Wu, Ren'an,Zou, Hanfa,&Liu, Yiwei.(2011).Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,25(13),1862-1868.
MLA	Sun, Shutao,et al."Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 25.13(2011):1862-1868.