Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay

doi:10.1016/j.bios.2010.09.026

CORC > 化学研究所 > 中国科学院化学研究所

	Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay
	Shen, Weizhi; Li, Mingzhu; Xu, Liang; Wang, Shutao; Jiang, Lei; Song, Yanlin; Zhu, Daoben
刊名	BIOSENSORS & BIOELECTRONICS
	2011-01-15
卷号	26 期号:5 页码:2165-2170
关键词	Photonic Crystal Fluoroimmunoassay Protein Detection Fluorescence Enhancement Background Interference
ISSN号	0956-5663
DOI	10.1016/j.bios.2010.09.026
英文摘要	There has been immense interest in both instruments and methods to enhance fluorescence signal and achieve highly sensitive fluoroimmunoassay (FIA). In this paper, we present a facile, low-cost and general method of biotinylated colloidal photonic crystal (PC) to improve the FIA of avidin (avidin FIA). The fluorescence signal intensity of the avidin FIA on the colloidal PC can be enhanced over two orders of magnitude relative to the control sample, attributed to the large surface area, resonance field and coherent scattering effect of the colloidal PC. The detection limit is shrunk to 1/69 of that of the control sample. Furthermore, the signal to interference ratio (S/I ratio) is increased because the band-edge induced fluorescence enhancement is wavelength-selective. The interference fluorescence does not go up proportionally while the signal is significantly enhanced by the colloidal PCs. It is believed that the colloidal PC modified with biotin can act as an effective material for a general and sensitive fluoroimmunoassay. (C) 2010 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000286904400060
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/71791]
专题	中国科学院化学研究所
通讯作者	Li, Mingzhu
作者单位	Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids,Lab New Mat, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Shen, Weizhi,Li, Mingzhu,Xu, Liang,et al. Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):2165-2170.
APA	Shen, Weizhi.,Li, Mingzhu.,Xu, Liang.,Wang, Shutao.,Jiang, Lei.,...&Zhu, Daoben.(2011).Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay.BIOSENSORS & BIOELECTRONICS,26(5),2165-2170.
MLA	Shen, Weizhi,et al."Highly effective protein detection for avidin-biotin system based on colloidal photonic crystals enhanced fluoroimmunoassay".BIOSENSORS & BIOELECTRONICS 26.5(2011):2165-2170.