Online Monitoring of the Aggregation and Fusion of DPPC/PA by Static and Dynamic Light Scattering

doi:10.3866/PKU.WHXB20100422

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	Online Monitoring of the Aggregation and Fusion of DPPC/PA by Static and Dynamic Light Scattering
	Deng Lin; Liang De-Hai
刊名	ACTA PHYSICO-CHIMICA SINICA
	2010-04-01
卷号	26 期号:4 页码:862-868
关键词	Liposome Vesicle Phase Transition Fusion Laser Light Scattering
ISSN号	1000-6818
DOI	10.3866/PKU.WHXB20100422
英文摘要	Static and dynamic light scattering were employed to monitor the temperature-induced aggregation and fusion processes of liposomes consisting of 1.2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and palmitic acid (PA) in a molar ratio of 1 2. From 25 to 41 degrees C, the size and the relative molecular mass of the liposome increased with increasing temperature indicating the occurrence of aggregation or fusion. Meanwhile, the size showed strong angular dependence, suggesting that the vesicle structure was at least partially destroyed upon aggregation During the subsequent cooling process, the size and relative molecular mass continued to increase On the basis of these results, we propose that the aggregation is caused by the adhesion of liposomes in the palmitic acid rich zones The formed aggregate had a structure between that of Mayers and inverted hexagonal (H-II) packing Upon aggregation, the ratio of fusion was small. Our results also indicated that the vesicle was kinetically stable Once the transition from the gel state to semi-inverted hexagonal (H-II) packing was triggered, the aggregation or fusion of the liposome proceeded automatically.
语种	英语
出版者	PEKING UNIV PRESS
WOS记录号	WOS:000276958400011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/71245]
专题	中国科学院化学研究所
通讯作者	Liang De-Hai
作者单位	Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Deng Lin,Liang De-Hai. Online Monitoring of the Aggregation and Fusion of DPPC/PA by Static and Dynamic Light Scattering[J]. ACTA PHYSICO-CHIMICA SINICA,2010,26(4):862-868.
APA	Deng Lin,&Liang De-Hai.(2010).Online Monitoring of the Aggregation and Fusion of DPPC/PA by Static and Dynamic Light Scattering.ACTA PHYSICO-CHIMICA SINICA,26(4),862-868.
MLA	Deng Lin,et al."Online Monitoring of the Aggregation and Fusion of DPPC/PA by Static and Dynamic Light Scattering".ACTA PHYSICO-CHIMICA SINICA 26.4(2010):862-868.