A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva | |
Wang, Dan; Zhuo, Jun-Qiao; Zhao, Mei-Ping | |
刊名 | TALANTA
![]() |
2010-06-30 | |
卷号 | 82期号:1页码:432-436 |
关键词 | Immunoglobulin a (Iga) Saliva Sample Competitive Enzyme-linked Immunosorbent Assay (Celisa) |
ISSN号 | 0039-9140 |
DOI | 10.1016/j.talanta.2010.04.040 |
英文摘要 | A simple competitive enzyme-linked immunosorbent assay (cELISA) was established for rapid measurement of secretory immunoglobulin A (sIgA) in saliva. The method was based on competitive reaction between the immobilized IgA and free IgA in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human IgA. In comparison with the conventionally used Sandwich ELISA, the cELISA is simpler, low-cost, and shows better reproducibility since it is not affected by the variation of capture antibodies from different batches. The assay time was also significantly reduced from more than 5 h to less than 3 h. Different curve-fitting models were compared, among which the fully specified logit-log model gave the best results. The linear working range and limit of detection were found to be 0.1-100 mu g mL(-1) and 0.05 mu g mL(-1), respectively. Matrix effects of saliva samples were investigated and a reasonable range of dilution factors were proposed. The developed method offers a very practical approach for high-throughput measurement of sIgA in saliva samples. (C) 2010 Elsevier B.V. All rights reserved. |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000279488900063 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/71059] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Zhao, Mei-Ping |
作者单位 | Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Dan,Zhuo, Jun-Qiao,Zhao, Mei-Ping. A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva[J]. TALANTA,2010,82(1):432-436. |
APA | Wang, Dan,Zhuo, Jun-Qiao,&Zhao, Mei-Ping.(2010).A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva.TALANTA,82(1),432-436. |
MLA | Wang, Dan,et al."A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva".TALANTA 82.1(2010):432-436. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论