Two-Dimensional Synchronous Correlation Spectroscopy for Probing Fluorescence Energy Transfer

doi:10.3964/j.issn.1000-0593(2010)05-1184-05

CORC > 化学研究所 > 中国科学院化学研究所

	Two-Dimensional Synchronous Correlation Spectroscopy for Probing Fluorescence Energy Transfer
	Huang Kun 1; Chen Jing 1; Liu Shao-xuan 1; Gao Xiu-xiang 1; Pan Qing-hua 1; Zhang Cheng-feng 1,2; Liu Yu-hai 1,2; Xia Jin-ming 1,3; Qi Jian 1; Xu Yi-zhuang 1
刊名	SPECTROSCOPY AND SPECTRAL ANALYSIS
	2010-05-01
卷号	30 期号:5 页码:1184-1188
关键词	Orthogonal 2d Synchronous Spectra Cross Peak Interaction Energy Transfer
ISSN号	1000-0593
DOI	10.3964/j.issn.1000-0593(2010)05-1184-05
英文摘要	In the present paper, the authors developed a new approach by constructing two-dimensional (2D) UV-Vis/fluorescence heterogeneous synchronous spectrum based on the orthogonal sample design scheme (OSD) developed in our previous works to characterize energy transfer among different lanthanide ions during the luminescence process. The authors use the EuCl(3)-NdCl(3), system as an example. The preliminary experimental results on the 2D synchronous spectra of EuCl(3)-NdCl(3) mixture solutions have demonstrated that cross peaks can be observed among the UV-Vis absorption bands from Nd(3+) and fluorescence emission hands from Eu(3+). The cross peaks in the 2D synchronous spectra of EuCl(3)-NdCl(3) mixture solutions manifested the interaction between the fluorescence emission from Eu(3+) and UV-Vis absorbance from Nd(3+), and therefore gives out experimental evidences for the occurrence of energy transfer between Eu(3+) and Nd(3+) ions. The cross peaks are not from the interaction between the solvent, water, and the solute, Eu(3+) or Ne ions. Mathematical analysis performed on 2D synchronous spectra using variable concentration as an external perturbation shows that the orthogonal sample design scheme is indispensable in removing the interfering cross peaks in 2D synchronous spectra. In fact, if the authors detect, respectively, the fluorescence emission spectra of pure Eu(3+) solutions and the UV-Vis absorbance spectra of pure Nd(3+) solutions, then use these spectra data to construct a series of synthesized spectra of an assumed mixture solution in which Eu(3+) and Ne are not mixed together, because Eu(3+) and Nd(3+) ions are spatially separated, there are no intermolecular interactions that should have occurred. Therefore, there are no cross-peaks that can be observed in the comparative 2D synchronous spectra. The cross peaks in 2D synchronous correlation spectra gives out a new approach to characterizing energy transfer among different lanthanide ions during the luminescence process.
语种	英语
出版者	OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS
WOS记录号	WOS:000277256200008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/70347]
专题	中国科学院化学研究所
通讯作者	Xu Yi-zhuang
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.Liaoning Univ Tradit Chinese Med, Shenyang 110032, Peoples R China
推荐引用方式 GB/T 7714	Huang Kun,Chen Jing,Liu Shao-xuan,et al. Two-Dimensional Synchronous Correlation Spectroscopy for Probing Fluorescence Energy Transfer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(5):1184-1188.
APA	Huang Kun.,Chen Jing.,Liu Shao-xuan.,Gao Xiu-xiang.,Pan Qing-hua.,...&Wu Jin-guang.(2010).Two-Dimensional Synchronous Correlation Spectroscopy for Probing Fluorescence Energy Transfer.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(5),1184-1188.
MLA	Huang Kun,et al."Two-Dimensional Synchronous Correlation Spectroscopy for Probing Fluorescence Energy Transfer".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.5(2010):1184-1188.