Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex

doi:10.1021/la904173j

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	Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex
	Pu, Fang 1; Hu, Dan 1; Ren, Jinsong 1; Wang, Shu 2; Qu, Xiaogang 1
刊名	LANGMUIR
	2010-03-16
卷号	26 期号:6 页码:4540-4545
ISSN号	0743-7463
DOI	10.1021/la904173j
英文摘要	By taking advantage of the large signal amplification through efficient fluorescence resonance energy transfer (FRET) from the conjugated polymer to the intercalating dye mediated by DNA, a new strategy for nuclease assay has been developed using conjugated polymer and DNA/intercalating dye complex. The discrimination of DNA before or after digestion by nuclease denotes the universal application of the approach, in which either dsDNA or ssDNA substrates could be used for detecting nuclease activity. This method can be extended to most nucleases by simply changing the Substrate DNA. The present method is label-free, rapid,and highly sensitive, with a detection limit Much better or at least comparable to previous reports. fit addition, this assay is easy to implement for Visual detection with the assistance of a UV transilluminator. We reason that a similar strategy can be adopted for the detection of other analytes.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000275226700111
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/69345]
专题	中国科学院化学研究所
通讯作者	Ren, Jinsong
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resources Utilizat, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Pu, Fang,Hu, Dan,Ren, Jinsong,et al. Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex[J]. LANGMUIR,2010,26(6):4540-4545.
APA	Pu, Fang,Hu, Dan,Ren, Jinsong,Wang, Shu,&Qu, Xiaogang.(2010).Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex.LANGMUIR,26(6),4540-4545.
MLA	Pu, Fang,et al."Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex".LANGMUIR 26.6(2010):4540-4545.