Characterizing DNA Condensation and Conformational Changes in Organic Solvents

doi:10.1371/journal.pone.0013308

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	Characterizing DNA Condensation and Conformational Changes in Organic Solvents
	Ke, Fuyou 1,2; Luu, Yen Kim 3; Hadjiargyrou, Michael 3; Liang, Dehai 1,2
刊名	PLOS ONE
	2010-10-11
卷号	5 期号:10
ISSN号	1932-6203
DOI	10.1371/journal.pone.0013308
英文摘要	Organic solvents offer a new approach to formulate DNA into novel structures suitable for gene delivery. In this study, we examined the in situ behavior of DNA in N, N-dimethylformamide (DMF) at low concentration via laser light scattering (LLS), TEM, UV absorbance and Zeta potential analysis. Results revealed that, in DMF, a 21bp oligonucleotide remained intact, while calf thymus DNA and supercoiled plasmid DNA were condensed and denatured. During condensation and denaturation, the size was decreased by a factor of 8-10, with calf thymus DNA forming spherical globules while plasmid DNA exhibited a toroid-like conformation. In the condensed state, DNA molecules were still able to release the counterions to be negatively charged, indicating that the condensation was mainly driven by the excluded volume interactions. The condensation induced by DMF was reversible for plasmid DNA but not for calf thymus DNA. When plasmid DNA was removed from DMF and resuspended in an aqueous solution, the DNA was quickly regained a double stranded configuration. These findings provide further insight into the behavior and condensation mechanism of DNA in an organic solvent and may aid in developing more efficient non-viral gene delivery systems.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000282748100030
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/68709]
专题	中国科学院化学研究所
通讯作者	Ke, Fuyou
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys, Beijing 100871, Peoples R China 3.SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11794 USA
推荐引用方式 GB/T 7714	Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,et al. Characterizing DNA Condensation and Conformational Changes in Organic Solvents[J]. PLOS ONE,2010,5(10).
APA	Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,&Liang, Dehai.(2010).Characterizing DNA Condensation and Conformational Changes in Organic Solvents.PLOS ONE,5(10).
MLA	Ke, Fuyou,et al."Characterizing DNA Condensation and Conformational Changes in Organic Solvents".PLOS ONE 5.10(2010).