Fluorescence Spectra and Protonation of Ofloxacin in Strong Acidic Solutions

doi:10.3964/j.issn.1000-0593(2009)05-1308-05

CORC > 化学研究所 > 中国科学院化学研究所

	Fluorescence Spectra and Protonation of Ofloxacin in Strong Acidic Solutions
	Li Hui-zhen 1,2; Zhang Cheng-feng 3; Gao Xiu-xiang 1; Huang Kun 1; Zhai Yu-jing 1; Xu Yi-zhuang 1; Wu Jin-guang 1
刊名	SPECTROSCOPY AND SPECTRAL ANALYSIS
	2009-05-01
卷号	29 期号:5 页码:1308-1312
关键词	Ofloxacin Quinolone Sulfuric Acid Fluorescence Spectrum Ultraviolet Spectrum Protonation
ISSN号	1000-0593
DOI	10.3964/j.issn.1000-0593(2009)05-1308-05
英文摘要	Fluorescence and UV-Vis spectra of ofloxacin (OFL)in sulfuric acid were studied. In the present paper, a new protonation state of OFL was observed. In hydrochloric acid, OFL produced bright green, fluorescence upon excitation by UV radiation. The maximal emission wavelength of OFL is about 505 nm. However, OFL produces violet fluorescence when dissolved in concentrated sulfuric acid. The maximal emission wavelength changes into 400 run. Further analysis demonstrated that the above changes arise from the variation of protonation states of OFL molecule. In dilute sulfuric acid, OFL accepted one proton, resulting in a protonation state that is similar to the OFL molecule dissolved HCl solution. The corresponding fluorescence band occurs at 505 nm. In concentrated sulfuric acid solution, OFL might accept additional protons. As a result, the size of the conjugated system is reduced and the fluorescence band exhibits a blue shift. In sulfuric acid of moderate concentrations, two bands at 505 and 400 nm respectively were found in the fluorescence emission spectra, indicating that OFL in two different protonation states coexists in the solution. In addition, both excitation band in excitation spectra and absorption bands in UV-Vis spectra exhibit red-shifted with the decrease in the concentration of sulfuric acid. Based on the above result, OFL can be used as a spectral probe to reflect the variation of H(+) in strong acid environment.
语种	英语
出版者	OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS
WOS记录号	WOS:000265756100037
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/67645]
专题	中国科学院化学研究所
通讯作者	Xu Yi-zhuang
作者单位	1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing 100871, Peoples R China 2.Henan Normal Univ, Coll Chem & Environm Sci, Xinxiang 453007, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Li Hui-zhen,Zhang Cheng-feng,Gao Xiu-xiang,et al. Fluorescence Spectra and Protonation of Ofloxacin in Strong Acidic Solutions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2009,29(5):1308-1312.
APA	Li Hui-zhen.,Zhang Cheng-feng.,Gao Xiu-xiang.,Huang Kun.,Zhai Yu-jing.,...&Wu Jin-guang.(2009).Fluorescence Spectra and Protonation of Ofloxacin in Strong Acidic Solutions.SPECTROSCOPY AND SPECTRAL ANALYSIS,29(5),1308-1312.
MLA	Li Hui-zhen,et al."Fluorescence Spectra and Protonation of Ofloxacin in Strong Acidic Solutions".SPECTROSCOPY AND SPECTRAL ANALYSIS 29.5(2009):1308-1312.