Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration

doi:10.1074/jbc.M808028200

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	Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration
	Wang, Qinli 1; Chen, Bo 2; Liu, Peng 1; Zheng, Maozhong 1; Wang, Yuqing 1; Cui, Sujuan 3; Sun, Daye 3; Fang, Xiaohong 2; Liu, Chun-Ming 1; Lucas, William J.4
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2009-05-01
卷号	284 期号:18 页码:12000-12007
ISSN号	0021-9258
DOI	10.1074/jbc.M808028200
英文摘要	Calmodulin (CaM) is a highly conserved intracellular calcium sensor. In plants, CaM also appears to be present in the apoplasm, and application of exogenous CaM has been shown to influence a number of physiological functions as a polypeptide signal; however, the existence and localization of its corresponding apoplasmic binding sites remain controversial. To identify the site(s) of action, a CaM-conjugated quantum dot (QD) system was employed for single molecule level detection at the surface of plant cells. Using this approach, we show that QD-CaM binds selectively to sites on the outer surface of the plasma membrane, which was further confirmed by high resolution transmission electron microscopy. Measurements of Ca(2+) fluxes across the plasma membrane, using ion-selective microelectrodes, demonstrated that exogenous CaM induces a net influx into protoplasts. Consistent with these flux studies, calcium-green-dextran and FRET experiments confirmed that applied CaM/QD-CaM elicited an increase in cytoplasmic Ca(2+) levels. These results support the hypothesis that apoplasmic CaM can act as a signaling agent. These findings are discussed in terms of CaM acting as an apoplasmic peptide ligand to mediate transmembrane signaling in the plant kingdom.
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000265494600032
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/67031]
专题	中国科学院化学研究所
通讯作者	Lin, Jinxing
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Mol Environm Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.Hebei Normal Univ, Inst Mol Cell Biol, Shijiazhuang 050016, Hebei Province, Peoples R China 4.Univ Calif Davis, Dept Plant Biol, Coll Biol Sci, Davis, CA 95616 USA
推荐引用方式 GB/T 7714	Wang, Qinli,Chen, Bo,Liu, Peng,et al. Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(18):12000-12007.
APA	Wang, Qinli.,Chen, Bo.,Liu, Peng.,Zheng, Maozhong.,Wang, Yuqing.,...&Lin, Jinxing.(2009).Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration.JOURNAL OF BIOLOGICAL CHEMISTRY,284(18),12000-12007.
MLA	Wang, Qinli,et al."Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration".JOURNAL OF BIOLOGICAL CHEMISTRY 284.18(2009):12000-12007.