Dynamic regional changes of extracellular ascorbic acid during global cerebral ischemia: Studied with in vivo microdialysis coupled with on-line electrochemical detection

doi:10.1016/j.brainres.2008.11.096

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	Dynamic regional changes of extracellular ascorbic acid during global cerebral ischemia: Studied with in vivo microdialysis coupled with on-line electrochemical detection
	Liu, Kun; Lin, Yuqing; Yu, Ping; Mao, Lanqun
刊名	BRAIN RESEARCH
	2009-02-09
卷号	1253 页码:161-168
关键词	Ascorbic Acid Regional Distribution Global Cerebral Ischemia In Vivo Microdialysis On-line Electrochemical Detection
ISSN号	0006-8993
DOI	10.1016/j.brainres.2008.11.096
英文摘要	The morphologic and functional outcomes of cerebral ischemia generally result from the acute neurochemical changes that occur 1 h after cerebral ischemia. As one of the small chemical species, ascorbic acid (AA) is involved in almost all kinds of neurochemical processes in acute cerebral ischemia. To understand the neurochemical processes in global cerebral ischemia, this study compares the dynamic regional changes of extracellular AA level, with in vivo microdialysis coupled with on-line electrochemical detection, in four different brain regions, I h after global cerebral ischemia induced by two-vessel occlusion (2-VO). The regional distribution of physiological AA levels in the microdialysates from striatum, cortex, dorsal hippocampus, and ventral hippocampus were 2.97 +/- 0.06, 3.98 +/- 0.09, 3.02 +/- 0.47, and 3.80 +/- 0.29 mu M, respectively. in 1 h after 2-VO cerebral ischemia, the microdialysate AA levels in the above four regions varied in a different manner; the striatum AA slowly decreased to 86.49 +/- 5.53% of the basal level (n=3, P<0.05). The AA levels in the cortex, dorsal hippocampus, and ventral hippocampus increased to 549.80 +/- 167.86% (n=3, P<0.05), 167.81 +/- 41.85 % (n=4, P<0.05) and 261.24 +/- 65.00% (n=3, P<0.05), in relation to their respective basal levels, respectively. The recorded spatiotemporal regional changes in the extracellular AA levels essentially reflect the intricate neurochemical changes during the acute period of global cerebral ischemia and may thus be useful for understanding the neurochemical processes of global cerebral ischemia. (C) 2008 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000263738600017
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66557]
专题	中国科学院化学研究所
通讯作者	Mao, Lanqun
作者单位	Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100191, Peoples R China
推荐引用方式 GB/T 7714	Liu, Kun,Lin, Yuqing,Yu, Ping,et al. Dynamic regional changes of extracellular ascorbic acid during global cerebral ischemia: Studied with in vivo microdialysis coupled with on-line electrochemical detection[J]. BRAIN RESEARCH,2009,1253:161-168.
APA	Liu, Kun,Lin, Yuqing,Yu, Ping,&Mao, Lanqun.(2009).Dynamic regional changes of extracellular ascorbic acid during global cerebral ischemia: Studied with in vivo microdialysis coupled with on-line electrochemical detection.BRAIN RESEARCH,1253,161-168.
MLA	Liu, Kun,et al."Dynamic regional changes of extracellular ascorbic acid during global cerebral ischemia: Studied with in vivo microdialysis coupled with on-line electrochemical detection".BRAIN RESEARCH 1253(2009):161-168.